Image processing device, imaging device, computer-readable storage medium, and image processing method

ABSTRACT

A reflected light image and a fluorescent light image of an observed region are acquired with an imaging system. A gradation level of the reflected light image is set on the basis of a feature value of the acquired reflected light image. The focus of the imaging system is controlled in accordance with the set gradation level. The fluorescent light image is corrected using a focus-controlled reflected light image of the observed region. Accordingly, the image of an object in the observed region from which the fluorescent light is generated is sharpened.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is based upon and claims the benefit of priority fromJapanese Patent Application No. 2009-077564, filed on Mar. 26, 2009, theentire contents of which are incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to an image processing device, an imagingdevice, a computer-readable storage medium, and an image processingmethod and particularly relates to an image processing device, animaging device, a computer-readable storage medium, and an imageprocessing method for processing a fluorescent light image based on thefluorescent light from an object.

2. Description of the Related Art

Endoscopes are used in the medical field to observe the internal organsof a subject. An elongated and flexible insertion unit of an endoscopeis inserted into the body cavity of a subject, such as a patient. Whitelight is applied to body tissue in the body cavity via the insertedflexible insertion unit. By receiving the light reflected from the bodytissue with an imaging unit in the flexible insertion unit, a whitelight image of the body tissue is captured. The white light image of thebody tissue is displayed on a display unit of the endoscope. A user,such as a doctor, observes the interior of the body cavity of thesubject using the white light image of the body tissue that is displayedon the display unit of the endoscope.

In the field of endoscopy, endoscopes have also been used recently thatallow fluorescence observation of an observed region, such as bodytissue, in the body cavity. A fluorescence-observation endoscope appliesexcitation light to body tissue in the body cavity via a flexibleinsertion unit that is inserted into the body cavity. The endoscopecaptures a fluorescent light image of the body tissue by receiving, withits imaging unit in the flexible insertion unit, light ofautofluorescence or drug fluorescence that is generated from the bodytissue due to the application of excitation light. A user, such as adoctor, visually checks the fluorescent light image of the body tissue,which is captured as described above, using the display unit of theendoscope, and performs fluorescence observation of the observed regionusing the fluorescent light image of the body tissue. There are alsoapparatuses that acquire a normalization image based on light emittedfrom an observed region, such as body tissue, due to the application oflight on the observed region, and that performs division regarding afluorescent light image of the observed region using the normalizationimage in order to generate a normalized fluorescent light image (see,Japanese Laid-open Patent Publication No. 2002-336187).

In the conventional fluorescence observation, when the luminance of afluorescent light image of an observed region, such as body tissue, iscorrected by a normalization process, a white light image of theobserved region is generally used as the normalization image and theluminance value of the fluorescent light image of the observed region isdivided by the luminance value of the white light image.

SUMMARY OF THE INVENTION

An image processing device according to an aspect of the presentinvention includes an image acquiring unit that acquires a reflectedlight image and a fluorescent light image of an observed region, thereflected and the fluorescent light images being captured by an imagingsystem; a feature value calculating unit that calculates a feature valueof the reflected light image acquired by the image acquiring unit; agradation level setting unit that sets a gradation level of thereflected light image on the basis of the feature value; a control unitthat controls a focus of the imaging system in accordance with thegradation level; and a corrected fluorescent light image generating unitthat generates a corrected fluorescent light image that is obtained bycorrecting the fluorescent light image using a focus-controlledreflected light image of the observed region that is acquired by theimage acquiring unit after the focus is controlled.

A computer-readable storage medium according to another aspect of thepresent invention stores therein an image processing program thatcontains instructions. The instructions cause a computer to performacquiring a reflected light image of an observed region based on lightthat is reflected from the observed region; calculating a feature valueof the reflected light image; setting a gradation level of the reflectedlight image on the basis of the feature mount; controlling a focus of animaging system for the reflected light image in accordance with thegradation level; acquiring a focus-controlled reflected light image ofthe observed region after the focus is controlled; and generating acorrected fluorescent light image that is obtained by correcting thefluorescent light image of the observed region, using thefocus-controlled reflected light image.

An image processing method according to still another aspect of thepresent invention includes acquiring a reflected light image of anobserved region based on light that is reflected from the observedregion; calculating a feature value of the reflected light image;setting a gradation level of the reflected light image on the basis ofthe feature mount; controlling a focus of an imaging system for thereflected light image in accordance with the gradation level; acquiringa focus-controlled reflected light image of the observed region afterthe focus is controlled; and generating a corrected fluorescent lightimage that is obtained by correcting the fluorescent light image of theobserved region, using the focus-controlled reflected light image.

The above and other features, advantages and technical and industrialsignificance of this invention will be better understood by reading thefollowing detailed description of presently preferred embodiments of theinvention, when considered in connection with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a block diagram schematically representing an example of aconfiguration of an endoscope according to a first embodiment of thepresent invention;

FIG. 2 is a schematic diagram representing an example of a configurationof a rotation filter according to the first embodiment of the presentinvention;

FIG. 3 is a schematic diagram representing an example of transmittanceproperties of the rotation filter according to the first embodiment ofthe present invention;

FIG. 4 is a flowchart illustrating a process procedure of an imageprocessing device according to the first embodiment of the presentinvention;

FIG. 5 is a schematic diagram for specifically explaining operations ofthe image processing device according to the first embodiment;

FIG. 6 is a block diagram schematically representing an example of aconfiguration of an endoscope according to a second embodiment of thepresent invention;

FIG. 7A is a schematic diagram specifically explaining a feature valuecalculation process and a gradation level setting process in a casewhere a white light image in which a vessel structure is relativelyconcentrated is captured in the second embodiment of the presentinvention;

FIG. 7B is a schematic diagram specifically explaining the feature valuecalculation process and the gradation level setting process in a casewhere a white light image in which the vessel structure is dispersed iscaptured in the second embodiment of the present invention;

FIG. 8 is a block diagram schematically representing an example of aconfiguration of an endoscope according to a third embodiment of thepresent invention;

FIG. 9 is a schematic diagram representing an example of transmittanceproperties of a filter of a light source device according to the thirdembodiment of the present invention;

FIG. 10 is a flowchart illustrating a process procedure of an imageprocessing device according to the third embodiment of the presentinvention; and

FIG. 11 is a schematic diagram representing an example of a peak-to-peakdifference of the color distribution in a white light image of anobserved region that can be calculated by a feature value calculator ofthe endoscope according to the second embodiment of the presentinvention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

Image processing devices, imaging devices, computer-readable storagemedia, and image processing methods according to some embodiments of thepresent invention are explanation in detail below with reference to theaccompanying drawings. In the following description, endoscopes thatcapture an image of the interior of the body cavity of a subject, suchas a patient, are explained as an example of an imaging device accordingto the present invention, and the image processing devices, thecomputer-readable storage media, and the image processing methods thatare used for the endoscopes are explained. The present invention,however, is not limited to the embodiments.

First Embodiment

FIG. 1 is a block diagram schematically representing an example of aconfiguration of an endoscope according to a first embodiment of thepresent invention. An endoscope 1 according to the first embodiment isan example of an imaging device according to the present invention. Asrepresented in FIG. 1, the endoscope 1 includes a light source device 10that applies light to an observed region 100 in a subject, such as apatient; an elongated insertion unit 20 that is inserted into the bodycavity of the subject; and an image processing device 30 that processesan image of the observed region 100.

The light source device 10 functions as a light source unit that appliesexcitation light for exciting a fluorescent agent and white light, whichis an example of illuminating light that illuminates an object to theobserved region 100. Specifically, the light source device 10 includes awhite light source 11; a collimating lens 12 that causes the lightemitted from the white light source 11 to be approximately parallellight; a condenser lens 13 that concentrates the collimated light; arotation filter 14 that switches the illuminating light to the observedregion 100 from excitation light to white light or vise versa; and amotor 15 that is a driver of the rotation filter 14. The light sourcedevice 10 has a function of switching the light emitted betweenexcitation light and white light alternately.

The white light source 11 is configured with a light source that canemit white light in a broadband covering the wavelength band of theexcitation light that excites the fluorescent agent. Specifically, thepower supply to the white light source 11 is turned on and off byoperating the switch (not shown) of the light source device 10, and thelight emitting timing is controlled by a controller 39 of the imageprocessing device 30 to be described below. The white light that isemitted by the white light source 11 contains color lights of a bluecolor component (B), a green color component (G), and a red colorcomponent (R), and further contains excitation light that excites afluorescent agent accumulated on a lesion 101, such as a tumor. Theexcitation light from the white light source 11 is of the wavelengthband of visible light or lower (for example, of the wavelength band ofultraviolet) and excites the fluorescent agent accumulated on the lesion101 to cause emission of fluorescent light in the wavelength band ofvisible light, for example, the wavelength band of 400 to 740 nm.

The collimating lens 12 is arranged in the optical path of the whitelight that is emitted from the white light source 11, and causes thewhite light from the white light source 11 to be approximately parallellight. The light that is collimated by the collimating lens 12 passesthrough the rotation filter 14, and then is concentrated by thecondenser lens 13. The light that is concentrated by the condenser lens13 is applied via the insertion unit 20 to the observed region 100 inthe subject.

The rotation filter 14 extracts light in a predetermined wavelength bandfrom the white light that is emitted by the white light source 11. FIG.2 is a schematic diagram representing an example of transmittanceproperties of the rotation filter. FIG. 3 illustrates a correlation lineC3 of wavelength with respect to intensity that illustrates the spectralproperties of the fluorescent light that is generated due to theexcitation light extracted by the rotation filter 14. As represented inFIG. 2, the rotation filter 14 includes a white light filter 14 a and anexcitation light filter 14 b with different spectral properties.

The white light filter 14 a allows white light in the predeterminedwavelength band out of the white light, which is emitted by the whitelight source 11, to pass. Specifically, the white light filter 14 a hastransmittance properties that allow the white light in a wavelength bandof 400 to 740 nm to pass as represented by the correlation line C1 ofwavelength with respect to intensity represented in FIG. 3. The whitelight filter 14 a with such transmittance properties extracts the whitelight in the wavelength band of 400 to 740 nm from the light emitted bythe white light source 11 and allows the extracted white light to passas the illuminating light to the observed region 100.

The excitation light filter 14 b allows the excitation light in thepredetermined wavelength band out of the white light that is emitted bythe white light source 11 to pass. Specifically, the excitation lightfilter 14 b has transmittance properties that allow light in thewavelength band of 400 nm or lower, for example, in the wavelength bandof ultraviolet as represented by the correlation line C2 of wavelengthwith respect to intensity. The excitation light filter 14 b with suchtransmittance properties extracts the excitation light in the wavelengthband illustrated by the correlation line C2 represented in FIG. 3 fromthe white light that is emitted by the white light source 11 and allowsthe extracted excitation light to pass. The excitation light that isextracted by the excitation light filter 14 b excites the fluorescentagent that is specifically accumulated on the lesion 101, such as atumor, existing in the observed region 100 to cause generation offluorescent light in the wavelength band of visible light, for example,in the wavelength band of 400 to 740 nm as illustrated by thecorrelation line C3 shown in FIG. 3.

The rotation filter 14 that includes the white light filter 14 a and theexcitation light filter 14 b is driven by the motor 15 to rotate in thecircumferential direction. This switches between the white light filter14 a and the excitation light filter 14 b and positions them alternatelyin the optical path of the white light from the white light source 11(see the dotted line in the light source device 10 represented in FIG.1). In the state where the white light filter 14 a is positioned in theoptical path, the rotation filter 14 allows the white light of 400 to740 nm to pass. In the state where the excitation light filter 14 b ispositioned in the optical path, the rotation filter 14 allows theexcitation light in the wavelength band of the white light to pass. Inother words, the rotation filter 14 allows white light and excitationlight to pass alternately.

The insertion unit 20 is an elongated flexible structure that can beinserted into the body cavity of the subject, and can be curved in adesired direction in response to the operation of an operating unit (notshown) of the endoscope 1. As represented in FIG. 1, the base side ofthe insertion unit 20 is connected to the light source device 10 and theimage processing device 30, and the insertion unit 20 includes a lightguide fiber 21 that guides the light emitted from the light sourcedevice 10 to the tip portion; and a lens 22 that diffuses the light thatis guided by the light guide fiber 21. The insertion unit 20 furtherincludes an imaging system 26 that captures a reflected light image anda fluorescent light image of the observed region 100.

The light guide fiber 21 is configured with optical fibers. The lightguide fiber 21 propagates the white light and the excitation light thatare emitted alternately by the light source device 10 to the tip portionof the insertion unit 20 sequentially. The white light and theexcitation light from the light source device 10 that are sequentiallyguided by the light guide fiber 21 are diffused sequentially by the lens22 and then are alternately applied to the observed region 100 in thesubject.

The white light from the light source device 10 that is applied to theobserved region 100 illuminates the observed region 100 and is reflectedon the observed region 100. When the lesion 101 on which the fluorescentagent is accumulated previously exists in the observed region 100, theexcitation light that is applied to the observed region 100 at thetiming different from that of the white light excites the fluorescentagent on the lesion 101 in order to cause generation of, for example,the fluorescent light in the wavelength band of the white light.

The imaging system 26 is used to capture a reflected light image and afluorescent light image of the observed region 100. The imaging system26 includes an objective lens 23 that concentrates the reflected lightor the fluorescent light from the observed region 100; a barrier filter24 that cuts off the excitation light out of the concentrated light fromthe observed region 100; and an imaging unit 25 that captures the whitelight image and the fluorescent light image of the observed region 100.

The objective lens 23 concentrates the reflected light and thefluorescent light from the observed region 100. Specifically, when thewhite light from the light source device 10 is applied to the observedregion 100, the objective lens 23 concentrates the white light reflectedfrom the observed region 100. When the excitation light from the lightsource device 10 is applied to the observed region 100, the objectivelens 23 concentrates the fluorescent light that is generated from theobserved region 100 (specifically, the lesion 101) and the excitationlight that is reflected from the observed region 100.

The barrier filter 24 is used to cut off the excitation light containedin the light from the observed region 100. Specifically, as the whitelight filter 14 a does, the barrier filter 24 has transmittanceproperties that allow light in the wavelength band of 400 to 740 nm asrepresented by the correlation line C1 shown in FIG. 3. When theexcitation light from the light source device 10 is applied to theobserved region 100, the barrier filter 24 with such transmittanceproperties cuts off the excitation light that is reflected from theobserved region 100 out of the light that is concentrated by theobjective lens 23, and allows the fluorescent light from the observedregion 100 to pass to the imaging unit 25. When the white light from thelight source device 10 is applied to the observed region 100, thebarrier filter 24 allows the reflected light from the observed region100 that is concentrated by the objective lens 23, i.e., the white lightof 400 to 740 nm that is reflected from the observed region 100, to passto the imaging unit 25.

The imaging unit 25 captures the reflected light image of the observedregion 100 based on the illuminating light that is reflected from theobserved region 100 due to application of the illuminating light and thefluorescent light image of the observed region 100 based on thefluorescent light that is generated from the observed region 100 due toapplication of the excitation light. Specifically, the imaging unit 25includes an imaging device 25 a, such as a CCD or a CMOS image sensor;and a movable optical system 25 b, such as a lens that forms an opticalimage of a subject on a light receiver of the imaging device 25 a. Underthe control of the controller 39 of the image processing device 30 to bedescribed below, the imaging unit 25 captures the reflected light imageand the fluorescent light image of the observed region 100 whileadjusting its focus.

The imaging device 25 a is a Bayer color imaging device that includes agroup of color filters with different spectral properties arranged onthe light receiver. The imaging device 25 a receives the light from theobserved region 100 that is imaged by the movable optical system 25 bvia the color filter group, and performs a photoelectric conversionprocess on the received light with respect to each pixel of each colorcomponent in order to generate video signals of the observed region 100.When the white light from the light source device 10 is applied to theobserved region 100, the imaging device 25 a receives the white lightfrom the observed region 100 that is imaged by the movable opticalsystem 25 b via the color filter group, thereby capturing the whitelight image, which is an example of the reflected light image of theobserved region 100. Each time the imaging device 25 a captures a whitelight image of the observed region 100, the imaging device 25 asequentially transmits video signals of each color componentconstituting the white light image of the observed region 100 to theimage processing device 30.

The imaging device 25 a has a function of capturing the fluorescentlight image based on the fluorescent light from the subject. In otherwords, when the excitation light from the light source device 10 isapplied to the observed region 100, the imaging device 25 a receives thefluorescent light from the observed region 100 that is imaged by themovable optical system 25 b via the color filter group, therebycapturing the fluorescent light image of the observed region 100. Eachtime the imaging device 25 a captures a fluorescent light image of theobserved region 100 as described above, the imaging device 25 asequentially transmits each video signal of the fluorescent light imageof the observed region 100 to the image processing device 30.

The color filter group arranged on the light receiver of the imagingdevice 25 a consists of a plurality of color filters with differentspectral properties, for example, a mosaic primary color filter thatincludes a plurality of blue light color filters that allow blue lightto pass, a plurality of a green light color filters that allow greenlight to pass, and a plurality of red light color filters that allow redlight to pass. The color filter group separates the white light or thefluorescent light from the observed region 100 with the color filters ofthe respective color components with respect to each pixel of theimaging device 25 a and allows the separated light of each colorcomponent to pass to each pixel of the imaging device 25 a. The colorcomponents of the respective color filters contained in the color filtergroup are not limited to blue, green, and red, and they may be, forexample, yellow, cyan, and magenta.

The movable optical system 25 b is configured with an optical system,such as a lens, and a movable lens frame. The movable optical system 25b is driven and controlled by the controller 39 of the image processingdevice 30. Specifically, the movable optical system 25 b is driven underthe control of the controller 39, so that the relative distance betweenthe imaging device 25 a and the lens can be changed. The focus of themovable optical system 25 b is controlled by the controller 39, and thusthe movable optical system 25 b focuses on the observed region 100. Withthe focus being on the observed region 100, the movable optical system25 b images the white light or the fluorescent light from the observedregion 100, which passes through the barrier filter 24, on the lightreceiver of the imaging device 25 a. In this case, the imaging device 25a captures the white light image or the fluorescent light image of theobserved region 100 that is being focused on.

The movable optical system 25 b can shift the focus from the observedregion 100 at a desired degree under the control of the controller 39.With the focus being shifted from the observed region 100 under thecontrol of the controller 39, the movable optical system 25 b images thewhite light from the observed region 100, which passes through thebarrier filter 24, on the light receiver of the imaging device 25 a. Inthis case, after the focus is controlled, the imaging device 25 acaptures a focus-controlled white light image of the observed region 100after the focus control (hereinafter, “adjusted image”). Thereafter, themovable optical system 25 b returns to the state where it focuses on theobserved region 100 under the control of the controller 39. The adjustedimage of the observed region 100 is one of a white light image based onwhite light from the observed region 100, and the gradation level of theadjusted image varies depending on the result of adjusting the focus ofthe movable optical system 25 b under the control of the controller 39.

The image processing device 30 processes the information of the imagesof the observed region 100 that is captured by the imaging device 26,and outputs the white light image and the fluorescent light image of theobserved region 100. Specifically, as represented in FIG. 1, the imageprocessing device 30 includes an image acquiring unit 31 that acquiresthe information of the images of the observed region 100, which arecaptured by the imaging unit 25 of the imaging system 26; and a storageunit 32 that stores the image information of the observed region 100that is acquired by the image acquiring unit 31. The image processingdevice 30 includes a feature value calculator 33 that calculates afeature value of the reflected light image of the observed region 100;and a gradation level setting unit 34 that sets the gradation level ofthe reflected light image of the observed region 100. The imageprocessing device 30 further includes a corrected fluorescent lightimage generator 35 that generates a corrected fluorescent light imagethat is obtained by correcting the luminance value of the fluorescentlight image of the observed region 100; a white light image generator 36that generates the white light image of the observed region 100; animage output unit 37 that outputs the white light image and thefluorescent light image of the observed region 100; an input unit 38;and the controller 39.

In the image processing device 30, the image acquiring unit 31 isconnected to the imaging unit 25, the storage unit 32, the feature valuecalculator 33, and the corrected fluorescent light image generator 35.The storage unit 32 is connected to the corrected fluorescent lightimage generator 35 and the white light image generator 36. The featurevalue calculator 33 is connected to the gradation level setting unit 34.The corrected fluorescent light image generator 35 and the white lightimage generator 36 are connected to the image output unit 37. Thecontroller 39 has bidirectional connection with the image acquiring unit31, the feature value calculator 33; the gradation level setting unit34, the corrected fluorescent light image generator 35, the white lightimage generator 36, the image output unit 37, and the input unit 38. Thecontroller 39 also has bidirectional direction with the light sourcedevice 10 and the imaging unit 25.

The image acquiring unit 31 acquires the information of the images ofthe observed region 100 that are captured by the imaging system 26.Specifically, each time the imaging unit 25 captures a white light imageof the observed region 100 with the focus being on the observed region100, the image acquiring unit 31 acquires each video signal of the whitelight image of the observed region 100 from the imaging unit 25. Eachvideo signal of the white light image is an analog signal and issequentially output from the imaging unit 25 to the image acquiring unit31 at a predetermined time interval. The image acquiring unit 31converts each video signal of the white light image, which is acquiredfrom the imaging unit 25, from an analog signal to a digital signal andperforms signal processes, such as a noise reduction process, on eachvideo signal after the digital conversion. The image acquiring unit 31sequentially transmits each video signal of the white light image afterthe signal processes to the storage unit 32 and the feature valuecalculator 33.

Each time the imaging unit 25 captures a fluorescent light image of theobserved region 100 with the focus being on the observed region 100, theimage acquiring unit 31 acquires each video signal of the fluorescentlight image of the observed region 100 from the imaging unit 25. Eachvideo signal of the fluorescent light image is an analog signal and issequentially output from the imaging unit 25 to the image acquiring unit31 at a predetermined time interval. The image acquiring unit 31converts each video signal of the fluorescent light image that isacquired from the imaging unit 25 from an analog signal to a digitalsignal, and performs the signal processes, such as the noise reductionprocess, on each video signal after the digital conversion. The imageacquiring unit 31 sequentially transmits each video signal of thefluorescent light image after the signal processes to the storage unit32.

After the controller 39 controls the focus of the imaging system 26, theimage acquiring unit 31 also acquires information of an image of theobserved region 100 with the shifted focus. Specifically, each time theimaging unit 25 captures a white light image of the observed region 100with the focus being shifted from the observed region 100 under thecontrol of the controller 39, the image acquiring unit 31 acquires eachvideo signal of the adjusted image of the observed region 100 that isthe focus-controlled white light image from the imaging unit 25. As inthe above case of the white light image, each video signal of theadjusted image is sequentially output at a predetermined time intervalfrom the imaging unit 25 to the image acquiring unit 31, and then isconverted to a digital signal by the image acquiring unit 31. The imageacquiring unit 31 performs the signal processes, such as the noisereduction process, on each video image signal of the adjusted imageafter the digital conversion, and sequentially transmits each videosignal of the adjusted image after the signal processes to the correctedfluorescent light image generator 35.

The storage unit 32 stores the image information that is acquired by theimage acquiring unit 31. Specifically, the storage unit 32 sequentiallyacquires each video signal of the white light image of the observedregion 100, which is processed by the image acquiring unit 31, and thenstores each acquired video signal of the white light image. The storageunit 32 also sequentially acquires each video signal of the fluorescentlight image of the observed region 100, which is processed by the imageacquiring unit 31, and stores each acquired video signal of thefluorescent light image. Each time the storage unit 32 acquires imageinformation from the image acquiring unit 31, the storage unit 32sequentially updates the existing image information to the acquiredimage information. The white light image and the fluorescent light imageof the observed region 100 that are stored in the storage unit 32 areinformation of the images that are acquired with the focus being theobserved region 100. Each video signal of the white light image in thestorage unit 32 is appropriately read by the white light image generator36. Each video signal of the fluorescent light image in the storage unit32 is appropriately read by the corrected fluorescent light imagegenerator 35.

The feature value calculator 33 calculates the feature value of thereflected light image of the observed region 100, which is acquired bythe image acquiring unit 31. Specifically, the feature value calculator33 is configured with, for example, a Laplacian filter. The featurevalue calculator 33 acquires each video signal of the white light imageof the observed region 100 from the image acquiring unit 31 and detectsthe edge that is contained in the white light image on the basis of eachacquired video signal. The edge in the white light image is a luminanceboundary region in the image. Specifically, the edge is a pixel regionwhere the difference in luminance with respect to the neighboring pixelsis large in accordance with an image of a line object, for example,vessels contained in the white light image. The feature value calculator33 performs an edge detection process for detecting the entire edgecontained in the white light image of the observed region 100, using theLaplacian filter. The feature value calculator 33 calculates edgeinformation that is an example of the feature value of the white lightimage on the basis of the white light image after the edge detectionprocess. The edge information of the white light image, which iscalculated by the feature value calculator 33, is information on theedge in the white light image. The edge information is, for example, thetotal luminance value of the entire edge that is contained in the whitelight image. The feature value calculator 33 transmits the edgeinformation that is calculated as the feature value of the white lightimage of the observed region 100 to the gradation level setting unit 34.

The feature value calculator 33 performs the edge detection processusing the Laplacian filter. However, the filter used for the edgedetection process is not limited to the Laplacian filter. In otherwords, the feature value calculator 33 may detects the edge in the whitelight image of the observed region 100 using methods other than theLaplacian filter process.

The gradation level setting unit 34 sets the gradation level of thereflected light image of the observed region 100. Specifically, thegradation level setting unit 34 includes a data table 34 a, such as alookup table, used for setting the gradation level of the reflectedlight image. The data table 34 a contains each range of edge informationof the white light image of the observed region 100, which edgeinformation is an example of the feature value of the reflected lightimage, and contains gradation levels that are different depending on theranges. Each gradation level in the data table 34 a increases with ashift of the range to an increase of the edge information, and itdecreases with a shift of the range to a decrease of the edgeinformation. The gradation level setting unit 34 acquires the edgeinformation of the white light image of the observed region 100 from thefeature value calculator 33, and selects a gradation level thatcorresponds to the acquired edge information with reference to the datatable 34 a. The gradation level setting unit 34 sets the selectedgradation level as the gradation level of the white light image of theobserved region 100. In this case, the gradation level setting unit 34increases the gradation level of the white light image with an increaseof the edge information, which is acquired from the feature valuecalculator 33, and reduces the gradation level of the white light imagewith a decrease of the edge information, which is acquired from thefeature value calculator 33. The gradation level setting unit 34transmits the result of setting the gradation level to the controller39.

The data table 34 a of the gradation level setting unit 34 may bepreviously stored in the gradation level setting unit 34. Alternatively,the data table 34 a may be input by the input unit 38 or may be saved orupdated in the gradation level setting unit 34 under the control of thecontroller 39.

The corrected fluorescent light image generator 35 generates thecorrected fluorescent light image that is obtained by correcting theluminance value of the fluorescent light image of the observed region100 that is captured by the imaging unit 25. Specifically, the correctedfluorescent light image generator 35 reads each video signal of thefluorescent light image of the observed region 100 from the storage unit32. The corrected fluorescent light image generator 35 performs signalprocesses, such as an interpolation process and a white balance process,on the read fluorescent light image with respect to each video signal inorder to generate the fluorescent light image of the observed region 100is generated. The corrected fluorescent light image generator 35 alsoacquires each video signal of the adjusted image of the observed region100 from the image acquiring unit 31. The corrected fluorescent lightimage generator 35 performs the signal processes, such as theinterpolation process and the white light balance process, on each videosignal of the acquired adjusted image, thereby generating the adjustedimage of the observed region 100.

The adjusted image of the observed region 100 is the white light imagewith gradation in accordance with the gradation level that is set by thegradation level setting unit 34, i.e., the white light image with thereduced edge corresponding to the image of the line object that isoriginally contained in the white light image. The adjusted image isclose to a uniform image without edge. The corrected fluorescent lightimage generator 35 performs division regarding the fluorescent lightimage of the observed region 100 using the adjusted image close to theuniform image in order to generate the corrected fluorescent light imageof the observed region 100. In this case, the corrected fluorescentlight image generator 35 divides the luminance value of the fluorescentlight image to be corrected by the luminance value of the adjusted imagewith respect to each corresponding pixel between the adjusted image andthe fluorescent light image of the observed region 100. Accordingly, theluminance value of each pixel of the fluorescent light image to becorrected is normalized. The normalization process allows the correctedfluorescent light image generator 35 to accurately correct thebrightness and darkness (the luminance value) in the fluorescent lightimage that vary depending on the imaging distance between the observedregion 100, which is the object, and the imaging system 26 (for example,the imaging unit 25) without influence of the line object that iscontained in the object. The corrected fluorescent light image generator35 sequentially transmits each video signal of the corrected fluorescentlight image of the observed region 100, which is generated as describedabove, to the image output unit 37.

In the corrected fluorescent light image of the observed region 100 thatis generated by the corrected fluorescent light image generator 35, thelesion 101 from which the fluorescent light is generated due toapplication of the excitation light is drawn in pixels with relativelyhigh luminance regardless of the imaging distance between the observedregion 100 and the imaging system 26.

The white light image generator 36 generates the white light image ofthe observed region 100 that is captured by the imaging unit 25.Specifically, the white light image generator 36 reads video signals ofeach color component of the white light image of the observed region 100from the storage unit 32. The white light image generator 36 performsthe interpolation process on the read video signals of each colorcomponent in order to generate a 3-CCD video signal that is obtained bycombining the color components with respect to each pixel-unit assemblyin the light receiver of the imaging unit 25. The white light imagegenerator 36 performs a color conversion process and the gradationconversion process on each 3-CCD video signal, which is generated asdescribed above, in order to generate the white light image of theobserved region 100. The white light image generator 36 sequentiallytransmits each video signal of the generated white light image of theobserved region 100 to the image output unit 37.

The image output unit 37 outputs the white light image and the correctedfluorescent light image of the observed region 100. Specifically, theimage output unit 37 is configured with a desired display, such as a CRTdisplay or a liquid crystal display. The image output unit 37 acquireseach video signal of the corrected fluorescent light image of theobserved region 100 from the corrected fluorescent light image generator35 and acquires each video signal of the white light image of theobserved region 100 from the white light image generator 36. The imageoutput unit 37 displays and outputs the corrected fluorescent lightimage of the observed region 100 on the basis of each video signal thatis acquired from the corrected fluorescent light image generator 35, anddisplays and outputs the white light image of the observed region 100 onthe basis of each video signal that is acquired from the white lightimage generator 36. In this case, the image output unit 37 may displaythe white light image and the corrected fluorescent light image of theobserved region 100 side by side, or may display them such that thecorrected fluorescent light image is superimposed on the white lightimage of the observed region 100.

The input unit 38 functions as an external interface unit of the imageprocessing device 30. The input unit 38 is configured with inputdevices, for example, a keyboard and a mouse. The input unit 38 inputsvarious types of information to the controller 39 in response to theinput operation by the user, such as a doctor or a nurse. The varioustypes of information that is input by the input unit 38 to thecontroller 39 includes, for example, instruction information forinstructing the controller 39 to control the operations of the lightsource device 10 or of the image processing device 30, information forsetting the mode in which a white light image or a fluorescent lightimage of the observed region 100 is captured, and information in thedata table 34 a that is stored in the gradation level setting unit 34.

The input unit 38 may include a power supply switch that switches on oroff the power supply to the image processing device 30, may include ashutter button for starting the image capturing operation, or mayinclude a mode switching button for switching between various modesincluding the image capturing mode. In this case, the controller 39controls the operation of the light source device 10 and the operationof the imaging unit 25.

The controller 39 controls each operation of the image acquiring unit31, the feature value calculator 33, the gradation level setting unit34, the corrected fluorescent light image generator 35, the white lightimage generator 36, the image output unit 37, and the input unit 38,which are constituents of the image processing device 30, and controlsinput and output of signals between these constituents. The controller39 also controls the light source device 10 and the imaging system 26.

Specifically, the controller 39 is configured with a storage unit 39 athat stores predetermined process programs including an image processingprogram, and a computer that executes the process programs in thestorage unit 39 a. The storage unit 39 a is a computer-readable storagemedium according to the first embodiment. On the basis of settinginformation that is input by the input unit 38, the controller 39 setsvarious image-capturing conditions, such as a mode in which a whitelight image or a fluorescent light image of the observed region 100 iscaptured, or a gain. The controller 39 drives and controls the movableoptical system 25 b of the imaging unit 25 such that it focuses on theobserved region 100, and controls the imaging device 25 a such that itcaptures the white light image and the fluorescent light image of theobserved region 100 in this focused state. The controller 39 alsocaptures the gradation level that is set by the gradation level settingunit 34, drives and controls the movable optical system 25 b on thebasis of the acquired gradation level in order to control the focus ofthe imaging unit 25 such that the focus is shifted from the observedregion 100 by an amount of shift in accordance with the gradation level.The controller 39 controls the imaging device 25 a such that it capturesthe adjusted image of the observed region 100 in the unfocused state.Thereafter, the controller 39 drives and controls the movable opticalsystem 25 b such that it returns to the state where it focuses on theobserved region 100.

The controller 39 controls the start of operation, completion ofoperation, and operation timing of each constituent of the imageprocessing device 30 on the basis of instruction information that isinput by the input unit 38. Specifically, the controller 39 controls theimage acquiring unit 31 such that it sequentially outputs each videosignal of the white light image, which is captured by the imaging unit25, to the storage unit 32 and the feature value calculator 33 afterconverting each video signal to a digital signal, and such that itsequentially outputs each video signal of the adjusted image, which iscaptured by the imaging unit 25, to the corrected fluorescent lightimage generator 35 after converting each video signal to a digitalsignal. The controller 39 further controls the image acquiring unit 31such that it sequentially outputs each video signal of the fluorescentlight image, which is captured by the imaging unit 25, to the storageunit 32 after converting each video signal to a digital signal. Thecontroller 39 also controls the process for calculating the featurevalue of the white light image that is performed by the feature valuecalculator 33, the process for setting the gradation level of the whitelight image that is performed by the gradation level setting unit 34,the process for generating the corrected fluorescent light image that isperformed by the corrected fluorescent light image generator 35, and theprocess for generating the white light image that is performed by thewhite light image generator 36. The controller 39 also controls theimage output unit 37 such that it displays and outputs the correctedfluorescent light image of the observed region 100, which is generatedby the corrected fluorescent light image generator 35, and the whitelight image of the observed region 100, which is generated by the whitelight image generator 36.

The controller 39 controls the light emitting operation of the whitelight source 11 of the light source device 10 and the filter switchingoperation of the rotation filter 14 on the basis of the instructioninformation that is input by the input unit 38. Specifically, thecontroller 39 causes the white light source 11 to emit white light andcontrols driving of the motor 15 to rotate that is connected to therotation filter 14 via the rotation shaft, thereby controlling drivingof the rotation filter 14 to rotate. The controller 39 thus causes thewhite light filter 14 a and the excitation light filter 14 b to bepositioned alternately in the optical path of the white light from thewhite light source 11 at a predetermined time interval. In this manner,the controller 39 controls switching of the rotation filter 14 in theoptical path. In addition, the controller 39 knows which of the whitelight filter 14 a and the excitation light filter 14 b is positioned inthe optical path on the basis of the rotation drive state, such as therotational speed of the motor 15. When the white light filter 14 a ispositioned in the optical path, the controller 39 controls the imagingunit 25 such that is captures the white light image or the adjustedimage of the observed region 100. When the excitation light filter 14 bis positioned in the optical path, the controller 39 controls theimaging unit 25 such that it captures the fluorescent light image of theobserved region 100.

The operations of the image processing device 30 according to the firstembodiment of the present invention are explained below. FIG. 4 is aflowchart illustrating the process procedure of the image processingdevice according to the first embodiment of the present invention. Theimage processing device 30 according to the first embodiment performsthe process procedure represented in FIG. 4 to display and output thewhite light image and the corrected fluorescent light image of theobserved region 100.

In other words, as represented in FIG. 4, the image processing device 30first acquires the white light image of the observed region 100 (stepS101). At step S101, the controller 39 controls the light source device10, the imaging system 26, and the image acquiring unit 31 such that thewhite light image of the observed region 100 that is being focused on isacquired.

Under the control of the controller 39, the light source device 10 emitsthe white light with the white light source 11 and positions the whitelight filter 14 a of the rotation filter 14 in the optical path of thewhite light in order to apply the white light of 400 to 740 nm to theobserved region 100. The imaging unit 25 of the imaging system 26focuses on the observed region 100 because of drive and control of themovable optical system 25 b by the controller 39, and receives the whitelight that is reflected from the observed region 100 in this focusedstate, thereby capturing the white light image of the observed region100. Under the control of the controller 39, the image acquiring unit 31acquires each video signal of the white light image of the observedregion 100 from the imaging unit 25 and transmits each acquired videosignal to the storage unit 32 and the feature value calculator 33 afterperforming the above-described signal processes. The storage unit 32acquires each video signal of the white light image of the observedregion 100, which is acquired from the image acquiring unit 31.

Subsequently, the image processing device 30 acquires the fluorescentlight image of the observed region 100 to be corrected (step S102). Atstep S102, the controller 39 controls the light source device 10, theimaging system 26, and the image acquiring unit 31 such that thefluorescent light image of the observed region 100 that is being focusedon is acquired.

Under the control of the controller 39, the light source device 10 emitsthe white light with the white light source 11, and positions theexcitation light filter 14 b of the rotation filter 14 in the opticalpath of the white light. Accordingly, the light source device 10 appliesthe excitation light with which the fluorescent light in the wavelengthband of the white light can be generated to the observed region 100(specifically, in the wavelength band of 400 to 740 nm) to the observedregion 100. With the focus being on the observed region 100, the imagingunit 25 of the imaging system 26 captures the fluorescent light image ofthe observed region 100 by receiving the fluorescent light that isgenerated from the observed region 100 due to application of theexcitation light. Under the control of the controller 39, the imageacquiring unit 31 acquires each video signal of the fluorescent lightimage of the observed region 100 from the imaging unit 25 and transmitseach acquired image signal to the storage unit 32 after performing theabove-described signal processes. The storage unit 32 stores each videosignal of the fluorescent light image of the observed region 100, whichis acquired from the image acquiring unit 31.

The image processing device 30 performs the process for calculating thefeature value of the white light image of the observed region 100 (stepS103). At step S103, the controller 39 controls the feature valuecalculator 33 such that it calculates the feature value of the whitelight image of the observed region 100, which is acquired at step S101.

Under the control of the controller 39, the feature value calculator 33detects the entire edge in the white light image of the observed region100 on the basis of each video signal of the white light image, which isacquired from the image acquiring unit 31. On the basis of the whitelight image after the edge detection process, the feature valuecalculator 33 calculates the edge information of the white light image,for example, the total luminance value of the entire edge in the whitelight image. The feature value calculator 33 transmits the calculatededge information of the white light image of the observed region 100 tothe gradation level setting unit 34.

Subsequently, the image processing device 30 performs the process forsetting the gradation level of the white light image of the observedregion 100 on the basis of the feature value that is calculated at stepS103 (step S104). At step S104, the controller 39 controls the gradationlevel setting unit 34 such that it sets the gradation level of the whitelight image of the observed region 100 on the basis of the feature valuethat is calculated at step S103.

Under the control of the controller 39, the gradation level setting unit34 acquires the edge information, which is calculated at step S103 bythe feature value calculator 33, and sets the gradation level of thewhite light image of the observed region 100 in accordance with theacquired edge information with reference to the data table 34 a. Thegradation level setting unit 34 transmits the set gradation level of thewhite light image of the observed region 100 to the controller 39.

Subsequently, the image processing device 30 performs the process foradjusting the focus of the white light image of the observed region 100on the basis of the gradation level that is set at step S104 (stepS105). At step S105, the controller 39 acquires the gradation level ofthe white light image of the observed region 100, which is set by thegradation level setting unit 34, and calculates the amount of shift ofthe focus from the observed region 100 on the basis of the acquiredgradation level. The controller 39 controls the focus of the imagingsystem 26 such that its focus is shifted from the observed region 100 bythe calculated amount of a shift.

Under the control of the controller 39, the imaging unit 25 of theimaging system 26 changes the relative distance between the imagingdevice 25 a and the lens of the movable optical system 25 b in order toshift the focus from the observed region 100 by the amount of a shift inaccordance with the gradation level that is set by the gradation levelsetting unit 34.

The image processing device 30 then acquires the adjusted image that isthe focus-controlled white light image of the observed region 100 inaccordance with the set gradation level (step S106). At step S106, thecontroller 39 controls the light source device 10, the imaging system26, and the image acquiring unit 31 such that the adjusted image of theobserved region 100 in the unfocused state in accordance with the setgradation level.

Under the control of the controller 39, the light source device 10 emitsthe white light with the white light source 11 and positions again thewhite light filter 14 a of the rotation filter 14 in the optical path ofthe white light. Accordingly, the light source device 10 applies thewhite light of 400 to 740 nm to the observed region 100. The imagingunit 25 of the imaging system 26 receives the white light that isreflected from the observed region 100 with the focus being adjusted atstep S105, thereby capturing the adjusted image of the observed region100. Under the control of the controller 39, the image acquiring unit 31acquires each video signal of the adjusted image of the observed region100 from the imaging unit 25 and transmits each acquired video signal tothe corrected fluorescent light image generator 35 after performing theabove-described signal processes.

The image processing device 30 perform the process for generating thecorrected fluorescent light image of the observed region 100 using theadjusted image of the observed region 100 that is acquired at step S106(step S107). At step S107, the controller 39 controls the correctedfluorescent light image generator 35 such that it generates thecorrected fluorescent light image that is obtained by correcting theluminance value of the fluorescent light image of the observed region100, which is acquired at step S102.

Under the control of the controller 39, the corrected fluorescent lightimage generator 35 performs the normalization process on the fluorescentlight image of the observed region 100 to be corrected, using theadjusted image of the observed region 100 that is acquired at step S106,thereby generating the corrected fluorescent light image of the observedregion 100. Specifically, the corrected fluorescent light imagegenerator 35 performs the signal processes on each video signal of thefluorescent light image of the observed region 100, which is read fromthe storage unit 32, thereby generating the fluorescent light image ofthe observed region 100. The corrected fluorescent light image generator35 performs the signal processes on each pixel of the adjusted image ofthe observed region 100, which is acquired from the image acquiring unit31, thereby generating the adjusted image of the observed region 100.The corrected fluorescent light image generator 35 divides the luminancevalue of the fluorescent light image to be corrected by the luminancevalue of the adjusted image, with respect to each corresponding pixelbetween the adjusted image and the fluorescent light image of theobserved region 100, thereby normalizing the luminance value of eachpixel of the fluorescent light image to be corrected. Accordingly, thecorrected fluorescent light image generator 35 generates the correctedfluorescent light image of the observed region 100 that is obtained bycorrecting the luminance value of the fluorescent light image to becorrected. The corrected fluorescent light image generator 35 transmitseach video signal of the generated fluorescent light image of theobserved region 100 to the image output unit 37.

Subsequently, the image processing device 30 performs the process foroutputting the image information of the observed region 100 (step S108).At step S108, the controller 39 controls the white light image generator36 such that it generates the white light image of the observed region100 and controls the image output unit 37 such that it displays thewhite light image and the corrected fluorescent light image of theobserved region 100 to be output.

Under the control of the controller 39, the white light image generator36 reads video signals of each color component of the white light imageof the observed region 100 from the storage unit 32, and performs theabove-described various signal processes on the read video signals ofeach color component, thereby generating the white light image of theobserved region 100. The white light image generator 36 transmits eachvideo signal of the generated white light image of the observed region100 to the image output unit 37. Under the control of the controller 39,the image output unit 37 displays and outputs the corrected fluorescentlight image of the observed region 100, which is acquired from thecorrected fluorescent light image generator 35, and the white lightimage of the observed region 100, which is acquired from the white lightimage generator 36.

Thereafter, when a process completion operation, such as a predeterminedoff operation, is performed (YES at step S109), the image processingdevice 30 completes the process. In this case, the input unit 38 inputsinstruction information for completing the process. On the basis of theinput instruction information, the controller 39 completes theoperations of the light source device 10, the imaging system 26, andeach constituent of the image processing device 30. In contrast, whenthe process completion operation is not performed (NO at step S109), theimage processing device 30 goes back to step S101 and repeats theprocess procedure from step S101 and the following steps. In this case,the controller 39 performs the process procedure from step S101 to stepS109 in order to appropriately control the light source device 10, theimaging system 26, and each constituent of the image processing device30.

The operations of the image processing device 30 based on the processprocedure from step S101 to S109 are specifically explained below,taking, as an example, a case where the image of the line object that iscontained in the white light image of the observed region 100 is animage of vessels. FIG. 5 is a schematic diagram for specificallyexplaining the operations of the image processing device according tothe first embodiment.

The image processing device 30 acquires a white light image Pw of theobserved region 100 at step S101 and acquires a fluorescent light imagePf of the observed region 100 at step S102. The white light image Pw iscaptured with the focus being on the observed region 100. As representedin FIG. 5, the white light image Pw contains an image IL of the lesion101 in the observed region 100 and an image IB of vessels that are theline object. In other words, the white light image Pw contains the edgethat forms the luminance contrast in accordance with the image IB of thevessels. On the other hand, the fluorescent light image Pf is capturedwith the focus being on the observed region 100, and contains the imageIL based on the fluorescent light that is generated from the lesion 101in the observed region 100.

The image processing device 30 that acquires the white light image Pwand the fluorescent light image Pf of the observed region 100 calculatesthe feature value of the white light image Pw and sets the gradationlevel of the white light image Pw on the basis of the calculated featurevalue. Specifically, at step S103, the feature value calculator 33detects the edge that is contained in the white light image Pw, i.e.,the edge that is the pixel region of which luminance is largelydifferent from that of the neighboring pixels in accordance with theimage IB of the vessels, and calculates the total luminance value of thedetected entire edge in the white light image Pw as the edgeinformation. On the other hand, the gradation level setting unit 34acquires the edge information of the white light image Pw that iscalculated by the feature value calculator 33, and sets the gradationlevel of the white light image Pw in accordance with the edgeinformation with reference to the data table 34 a. The gradation levelsetting unit 34 increases the gradation level with an increase of thetotal luminance value of the entire edge in the white light image Pw,which total luminance value is the edge information, and reduces thegradation level with a decrease in the total luminance value of theentire edge.

After setting the gradation level of the white light image Pw asdescribed above, the image processing device 30 acquires the adjustedimage Pr that is the white light image of the observed region 100 withgradation in accordance with the gradation level. Specifically, at stepsS105 and S106, the controller 39 calculates the amount of shift of thefocus in accordance with the gradation level of the white light image Pwand controls the imaging unit 25 such that it shifts the focus from theobserved region 100 by the calculated shift amount. Furthermore, thecontroller 39 controls the light source device 10 and the imaging unit25 such that the white light image of the observed region 100 in theunfocused state is acquired. Under the control of the controller 39, thelight source device 10 applies the white light to the observed region100 and the imaging unit 25 captures the focus-controlled white lightimage of the observed region 100 in the unfocused state in accordancewith the gradation level, i.e., captures the adjusted image Pr. Theimage processing device 30 acquires the adjusted image Pr of theobserved region 100 that is captured by the imaging unit 25.

The image processing device 30 that acquires the adjusted image Pr ofthe observed region 100 then corrects the luminance value of thefluorescent light image Pf of the observed region 100 using the adjustedimage Pr. Specifically, at step S107, the corrected fluorescent lightimage generator 35 performs the normalization process on the fluorescentlight image Pf of the observed region 100 using the adjusted image Pr ofthe observed region 100, thereby generating a corrected fluorescentlight image Pc of the observed region 100. In the normalization process,the corrected fluorescent light image generator 35 divides the luminancevalue of the fluorescent light image Pf by the luminance value of theadjusted image Pr, with respect to each corresponding pixel between theadjusted image Pr and the fluorescent light image Pf, therebynormalizing the luminance value of each pixel of the fluorescent lightimage Pf.

The adjusted image Pr of the observed region 100 is the white lightimage with gradation in accordance with the gradation level that is setby the gradation level setting unit 34. The adjusted image Pr hasreduced luminance contrast due to the edge corresponding to the image IBof the vessels, which are the line object in the white light image Pw ofthe observed region 100. Accordingly, the adjusted image Pr is close tothe uniform image without edge (i.e., without luminance contrast). Thecorrected fluorescent light image generator 35 normalizes the luminancevalue of each pixel of the fluorescent light image Pf using the adjustedimage Pr. Accordingly, the corrected fluorescent light image generator35 generates the corrected fluorescent light image Pc that is obtainedby accurately correcting the brightness and darkness (the luminancevalue) in the fluorescent light image Pf that vary depending on theimaging distance between the observed region 100 and the imaging system26 without influence of the edge (luminance contrast) corresponding tothe image IB of the vessels that are the line object.

Thereafter, as represented in FIG. 5, the image processing device 30displays the white light image Pw and the corrected fluorescent lightimage Pc of the observed region 100 on the image output unit 37. In thecorrected fluorescent light image Pc that is displayed on the imageoutput unit 37, the image IL of the lesion 101 from which thefluorescent light is generated due to application of the excitationlight is drawn in pixels with relatively high luminance regardless ofthe imaging distance between the observed region 100 and the imagingsystem 26. This increases the capability of detecting the lesion 101 inthe subject by fluorescence observation of the observed region 100 andincreases the diagnostic capability.

As described above, in the first embodiment of the present invention,first acquired are the white light image of the observed region based onthe white light, which is reflected from the observed region due toapplication of the white light, and the fluorescent light image of theobserved region based on the fluorescent light, which is generated fromthe observed region due to application of the excitation light. Thefeature value concerning the edge of the acquired white light image iscalculated. On the basis of the calculated feature value, the gradationlevel of the white light image is set. The focus control for shiftingthe focus from the observed region is performed in accordance with theset gradation level, and then the adjusted image that is thefocus-controlled white light image of the observed region is acquired.Division regarding the fluorescent light image of the observed region isperformed using the adjusted image in order to generate the correctedfluorescent light image that is obtained by correcting the luminancevalue of the fluorescent light image. Therefore, even if the white lightimage of the observed region contains an image of a line object, such asvessels, the luminance contrast in the white light image caused by theimage of the line object can be reduced by appropriately gradating thewhite light image. Accordingly, the luminance value of the fluorescentlight image of the observed region can be normalized using the adjustedimage of the observed region that is close to the uniform image withoutluminance contrast caused by edge. Thus, the luminance of thefluorescent light image of the observed region can be corrected withhigh accuracy without influence of the line object in the observedregion.

By use of the image processing device, the imaging device, thecomputer-readable storage medium, and the image processing method,abnormal body tissue, such as a lesion, in the observed region can beclearly drawn in the fluorescent light image. This increases thecapability of detecting abnormal body tissue in the subject using thefluorescent light image to be observed and increases the diagnosticcapability.

Second Embodiment

A second embodiment of the present invention is explained below. In thefirst embodiment, the feature value calculator 33 calculates the edgeinformation of the white light image of the observed region 100 as thefeature value of the white light image. In the second embodiment, thecolor distribution information of a white light image of the observedregion 100 is calculated as a feature value of the white light image.

FIG. 6 is a block diagram schematically representing an example of aconfiguration of an endoscope according to the second embodiment of thepresent invention. As represented in FIG. 6, an endoscope 2 according tothe second embodiment includes an image processing device 230 instead ofthe image processing device 30 according to the first embodiment. Theimage processing device 230 includes a feature value calculator 233instead of the feature value calculator 33 of the image processingdevice 30 according to the first embodiment, a gradation level settingunit 234 instead of the gradation level setting unit 34; and acontroller 239 instead of the controller 39. Other aspects of theconfiguration of the endoscope 2 are same as those of the firstembodiment, and the same constituents are denoted by the same referencenumbers.

The image processing device 230 includes the feature value calculator233, the gradation level setting unit 234, and the controller 239 asdescribed above. The image processing device 230 calculates the colordistribution information of the white light image of the observed region100 as the feature value of the white light image, and sets a gradationlevel of the white light image on the basis of the calculated colordistribution information. The image processing device 230 has functionssimilar to those of the image processing device 30 according to thefirst embodiment in addition to the functions of the feature valuecalculator 233, the gradation level setting unit 234, and the controller239.

The feature value calculator 233 calculates the color distributioninformation of the white light image of the observed region 100, whichis captured by the imaging unit 25, as the feature value of thereflected light image of the observed region 100. Specifically, thefeature value calculator 233 acquires each video signal of the whitelight image of the observed region 100 from the image acquiring unit 31and detects a predetermined color component that is contained in thewhite light image on the basis of each acquired video signal. Thefeature value calculator 233 calculates dispersion that represents thedegree of dispersion of the detected color component in the white lightimage. The dispersion of the predetermined color component that iscalculated by the feature value calculator 233 is an example of thecolor distribution information as the feature value of the white lightimage of the observed region 100. The dispersion increases with anincrease of the degree of dispersion (i.e., a decrease of the degree ofconcentration) of the predetermined color component in the white lightimage and decreases with a decrease of the dispersion degree (i.e., anincrease of the concentration degree) of the predetermined colorcomponent in the white light image. The feature value calculator 233transmits the calculated color distribution information of the whitelight image of the observed region 100 to the gradation level settingunit 234.

The color component of the color distribution information that iscalculated by the feature value calculator 233 may be a color componentthat has, or contained in, the largest share among the color componentsthat are contained in the white light image of the observed region 100,which is acquired from the image acquiring unit 31. Alternatively, thecolor component may be a color component of a pixel region (i.e., edge)corresponding to a line object, such as vessels, that is contained inthe white light image.

The gradation level setting unit 234 includes a data table 234 a insteadof the data table 34 a according to the first embodiment. The gradationlevel setting unit 234 sets the gradation level of the reflected lightimage of the observed region 100 with reference to the data table 234 a.The data table 234 a contains each range of dispersion (dispersiondegree) of the predetermined color that is an example of the colordistribution information of the white light image of the observed region100 and the gradation level that differs in each range of dispersion ofthe predetermined color in the white light image. Each gradation levelin the data table 234 a decreases with a shift of the range to anincrease of the dispersion of the predetermined color in the white lightimage, and it increases with a shift of the range to a decrease of thedispersion of the predetermined color in the white light image. Thegradation level setting unit 234 acquires the color distributioninformation of the white light image of the observed region 100 from thefeature value calculator 233, and selects a gradation level thatcorresponds to the acquired color distribution information withreference to the data table 234 a. The gradation level setting unit 234sets the selected gradation level as the gradation level of the whitelight image of the observed region 100. In this case, the gradationlevel setting unit 234 increases the gradation level of the white lightimage of the observed region 100 with a decrease of the colordistribution information, which is acquired from the feature valuecalculator 233, i.e., a decrease of the dispersion of the predeterminedcolor in the white light image. The gradation level setting unit 234also reduces the gradation level of the white light image of theobserved region 100 with an increase of the color distributioninformation, which is acquired from the feature value calculator 233,i.e., an increase of the dispersion of the predetermined color in thewhite light image. The gradation level setting unit 234 transmits theresult of setting the gradation level to the controller 239.

The data table 234 a of the gradation level setting unit 234 may bepreviously stored in the gradation level setting unit 234.Alternatively, the data table 234 a may be input by the input unit 38and saved or updated in the gradation level setting unit 234 under thecontrol of the controller 239.

The controller 239 is configured with a storage unit 239 a that storespredetermined process programs including an image processing program,and a computer that executes the process programs in the storage unit239 a. The storage unit 239 a is a computer-readable storage mediumaccording to the second embodiment. The controller 239 controls thefeature value calculator 233 such that it calculates, as the featurevalue of the white light image of the observed region 100, the colordistribution information of the white the white light image, forexample, the dispersion of the predetermined color component in thewhite light image, and controls the gradation level setting unit 234such that it sets the gradation level of the white light image using thecalculated color distribution information of the white light image. Thecontroller 239 has functions similar to those of the controller 39 ofthe image processing device 30 according to the first embodiment inaddition to the function of controlling the feature value calculator 233and the gradation level setting unit 234.

The operations of the image processing device 230 according to thesecond embodiment of the present invention are explained below. FIG. 7Aand FIG. 7B are schematic diagrams specifically explaining respectivelya feature value calculation process and a gradation level settingprocess on the white light image according to the second embodiment ofthe present invention. The image processing device 230 according to thesecond embodiment performs operations similar to those of the imageprocessing device 30 according to the first embodiment in addition tothe feature value calculation process and the gradation level settingprocess on the white light image of the observed region 100. In otherwords, the image processing device 230 performs a process proceduresimilar to that of the image processing device 30 according to the firstembodiment in addition to the process procedure of steps S103 and S104out of steps S101 to S109 represented in FIG. 4.

The feature value calculation process and the gradation level settingprocess that are performed by the image processing device 230 on thewhite light image of the observed region 100 are specifically explainedbelow with reference to FIG. 7A and FIG. 7B taking, as an example, acase where the white light image of the observed region 100 is imageinformation that consists of each pixel of the blue color component,each pixel of the green color component, and each pixel of the red colorcomponent, and where the color component of vessels (i.e., redcomponent) that is a line object contained in the white light image isdetected.

The feature value calculation process and the gradation level settingprocess in a case where the image processing device 230 acquires a whitelight image Pw1 in which the vessel structure is relatively concentratedas represented in FIG. 7A are explained below. At step S103, the featurevalue calculator 233 acquires each video signal of the white light imagePw1 of the observed region 100 from the image acquiring unit 31, anddetects the red color component that is the color component of thevessels in the white light image Pw1 on the basis of each acquired videosignal. The feature value calculator 233 calculates the degree ofdispersion of the pixels of the detected red color component in thewhite light image Pw1 as the dispersion of the red color component inthe white light image Pw1. The feature value calculator 233 transmitsthe dispersion of the red color component in the white light image Pw1,which is calculated as described above, to the gradation level settingunit 234.

An image IB1 of the vessels that are the line object in the white lightimage Pw1 exists and is relatively concentrated in the image asrepresented in FIG. 7A. In this case, the dispersion of the red colorcomponent, which is calculated by the feature value calculator 233, is arelatively small value in accordance with the degree of theconcentration of the vessels in the white light image Pw1.

At step S104, the gradation level setting unit 234 acquires the resultof calculating the dispersion of the red color component in the whitelight image Pw1 from the feature value calculator 233. The gradationlevel setting unit 234 sets the gradation level in accordance with theacquired dispersion of the red color component in the white light imagePw1 as the gradation level of the white light image Pw1 with referenceto the data table 234 a. The dispersion of the red color component inthe white light image Pw1 is relatively small as described above, i.e.,the degree of concentration of the red color component in the whitelight image Pw1 is relatively large. In this case, the gradation levelsetting unit 234 sets relatively higher the gradation level of the whitelight image Pw1 in accordance with the small the small dispersion of thered color component in the white light image Pw1. Accordingly, at stepS106, the image processing device 230 acquires an adjusted image Pr1 ofthe observed region 100 with the focus being shifted in accordance withthe gradation level of the white light image Pw1.

The adjusted image Pr1 of the observed region 100 is a white light imagethat is gradated in a large extent in accordance with the gradationlevel (high) of the white light image Pw1, i.e., the image IB1 of thevessels that are the line object contained in the image is sufficientlygradated. This reduces the entire edge corresponding to the vessels inthe adjusted image Pr1, and accordingly, sufficiently reduces thecontrast in the adjusted image Pr1 resulting from the edge correspondingto the image IB1 of the vessels, so that the adjusted image Pr1 is closeto the uniform image without contrast due to edge. As in the case of thefirst embodiment, the corrected fluorescent light image generator 35 ofthe image processing device 230 generates the corrected fluorescentlight image of the observed region 100 by performing the normalizationprocess on the fluorescent light image of the observed region 100 usingthe adjusted image Pr1. In the corrected fluorescent light image, animage IL1 of the lesion from which the fluorescent light is generated isdrawn in pixels with relatively high luminance.

The feature calculation process and the gradation level setting processin a case where the image processing device 230 acquires a white lightimage Pw2 in which the vessel structure is relatively dispersed asrepresented in FIG. 7B are explained below. At step S103, the featurevalue calculator 233 acquires each video signal of the white light imagePw2 of the observed region 100 from the image acquiring unit 31, anddetects the red color component that is the color component of thevessels in the white light image Pw2 on the basis of each acquired videosignal. The feature value calculator 233 calculates the degree ofdispersion of the pixels of the detected red color component in thewhite light image Pw2 as the dispersion of the red color component inthe white light image Pw2. The feature value calculator 233 transmitsthe dispersion of the red color component in the white light image Pw2,which is calculated as described above, to the gradation level settingunit 234.

An image IB2 of the vessels that are the line object in the white lightimage Pw2 exists and dispersed in the relatively-entire image asrepresented in FIG. 7B. In this case, the dispersion of the red colorcomponent, which is calculated by the feature value calculator 233, is arelatively large value, compared to that of the white light image Pw1,in accordance with the degree of dispersion of the vessels in the whitelight image Pw2.

At step S104, the gradation level setting unit 234 acquires the resultof calculating the dispersion of the red color component in the whitelight image Pw2 from the feature value calculator 233. The gradationlevel setting unit 234 sets the gradation level in accordance with theacquired dispersion of the red color component in the white light imagePw2 as the gradation level of the white light image Pw2 with referenceto the data table 234 a. The dispersion of the red color component inthe white light image Pw2 is relatively large as described above (e.g,larger than that of the white light image Pw1), i.e., the degree ofconcentration of the red color component in the white light image Pw2 isrelatively small. In this case, the gradation level setting unit 234sets relatively lower the gradation level of the white light image Pw2(lower than that of the white light image Pw1) in accordance with thelarge dispersion of the red color component in the white light imagePw2. Accordingly, at step S106, the image processing device 230 acquiresan adjusted image Pr2 of the observed region 100 with the focus beingshifted in accordance with the gradation level of the white light imagePw2.

The adjusted image Pr of the observed region 100 is a white light imagethat is gradated in a small extent in accordance with the gradationlevel (low) of the white light image Pw2, i.e., the image IB2 of thevessels that are the line object contained in the image is appropriatelygradated. This sufficiently reduces the edge corresponding to thevessels in the adjusted image Pr, and accordingly, sufficiently reducesthe contrast in the adjusted image Pr resulting from the edgecorresponding to the image IB2 of the vessels, so that the adjustedimage Pr is close to the uniform image without contrast due to edge. Asin the case of the first embodiment, the corrected fluorescent lightimage generator 35 of the image processing device 230 generates thecorrected fluorescent light image of the observed region 100 byperforming the normalization process on the fluorescent light image ofthe observed region 100 using the adjusted image Pr. In the correctedfluorescent light image, an image IL2 of the lesion from which thefluorescent light is generated is drawn in pixels with relatively highluminance.

As explained above, in the second embodiment of the present invention,the feature value concerning the acquired color distribution of thewhite light image of the observed region is calculated. On the basis ofthe calculated feature value, the gradation level of the white lightimage is set. Other aspects of the configuration are similar to those ofthe first embodiment. Thus, functions and effects similar to those ofthe first embodiment are achieved. Particularly, the correctedfluorescent light image that is preferable to fluorescence observationof the human body with a complicated vessel structure as an line objectcan be generated and output.

Third Embodiment

A third embodiment of the present invention is explained below. In thefirst embodiment, the fluorescent light image and the adjusted image ofthe observed region 100 are captured at different timing. In the thirdembodiment, the fluorescent light image and the adjusted image of theobserved region 100 are captured at the same timing.

FIG. 8 is a block diagram schematically representing an example of aconfiguration of an endoscope according to the third embodiment of thepresent invention. As represented in FIG. 8, an endoscope 3 according tothe third embodiment includes a light source device 310 instead of thelight source device 10 according to the first embodiment, an imagingsystem 328 instead of the imaging system 26; and an image processingdevice 330 instead of the image processing device 30. In the thirdembodiment, the light source device 310 includes a white light source311 instead of the white light source 11 of the light source device 10according to the first embodiment; and a fixed filter 314 instead of therotation filter 14 and the motor 15. The imaging system 328 includes abarrier filter 324 instead of the barrier filter 24 of the imagingsystem 26 according to the first embodiment; and a combined imaging unit325 instead of the single imaging unit 25, and further includes a lightseparator that separates the light from the observed region 100. Theimage processing device 330 includes a controller 339 instead of thecontroller 39 of the image processing device 30 according to the firstembodiment. Other aspects of the configuration of the endoscope 3 aresame as those of the first embodiment. The same constituents are denotedby the same reference numbers.

The light source device 310 functions as a light source unit thatapplies excitation light for exciting a fluorescent agent and whitelight, which is an example of illuminating light that illuminates anobject, to the observed region 100. Specifically, the light sourcedevice 310 includes the collimating lens 12; the condenser lens 13; thewhite light source 311 that can emit white light in a broadband coveringa predetermined wavelength band of excitation light; and the filter 314that allows light in a predetermined wavelength to pass. The lightsource device 310 has a function of simultaneously applying theexcitation light and the white light to the observed region 100.

The white light source 311 is achieved with a light source that can emitthe white light in the broadband covering the wavelength band of theexcitation light that excites the fluorescent agent. The power supply tothe white light source 311 is turned on or off by operating the switch(not shown) of the light source device 310 and the light emitting timingis controlled by the controller 339 of the image processing device 330.The white light source 311 emits the broadband white light in thewavelength band, for example, of 400 to 740 nm. The white light emittedby the white light source 311 contains color lights of the blue colorcomponent (B), the green color component (G), and the red colorcomponent (R), and further contains the excitation light in a wavelengthband of 680 to 740 nm that excites the fluorescent agent, for example,Cy7, accumulated on the lesion 101, such as a tumor. The excitationlight from the white light source 311 has a feature of exciting thefluorescent agent accumulated on the lesion 101 in order to causeemission of fluorescent light in a wavelength band, for example, of 760to 850 nm out of the wavelength band of visible light.

The filter 314 extracts light in the predetermined wavelength band fromthe white light that is emitted by the white light source 311. FIG. 9 isa schematic diagram representing an example of transmittance propertiesof the filter of the light source device according to the thirdembodiment of the present invention. FIG. 9 also illustrates theintensity properties of the fluorescent light that is generated due tothe excitation light that is extracted by the filter 314 of the lightsource device 310. The filter 314 has transmittance properties thatallow the white light in the wavelength band of 400 to 740 nm to pass asrepresented by the correlation line C1 of wavelength with respect totransmittance represented in FIG. 9. The filter 314 extracts the whitelight in the wavelength band of 400 to 740 nm from the light emitted bythe white light source 311 and allows the extracted white light to passas the illuminating light to the observed region 100.

The white light that is extracted by the filter 314 contains theexcitation light in the wavelength band of 680 to 740 nm, which excitesthe fluorescent agent (for example, Cy7) accumulated on the lesion 101in the observed region 100. In other words, the filter 314 allows thewhite light in the broadband covering the wavelength band of theexcitation light to pass. The excitation light in the wavelength band of680 to 740 nm that passes through the filter 314 causes generation ofthe fluorescent light in the wavelength band of 760 to 850 nm asrepresented by the correlation line C4 of wavelength with respect tointensity represented in FIG. 9. The white light and the excitationlight that are emitted by the light source device 310 with such aconfiguration are applied simultaneously to the observed region 100 viathe light guide fiber 21 of the insertion unit 20.

In the third embodiment, the insertion unit 20 includes the light guidefiber 21 and the lens 22, and further includes the imaging system 328that captures the reflected light image and the fluorescent light imageof the observed region 100. The imaging system 328 includes theobjective lens 23, a light separator 323, the barrier filter 324, andthe combined imaging unit 325. The combined imaging unit 325 includes awhite light imaging unit 326 that captures the white light image and theadjusted image of the observed region 100; and a fluorescent lightimaging unit 327 that captures the fluorescent light image of theobserved region 100.

The white light from the light source device 310 that is applied to theobserved region 100 via the light guide fiber 21 and the lens 22 of theinsertion unit 20 illuminates the observed region 100 and is reflectedon the observed region 100. Simultaneously, the excitation light that iscontained in the white light is reflected on the observed region 100 andexcites the fluorescent agent on the lesion 101 to cause generation offluorescent light in a wavelength band, for example, of 760 to 850 nm.The objective lens 23 concentrates the white light and the excitationlight that are reflected from the observed region 100 and thefluorescent light that is generated from the observed region 100(specifically, the lesion 101).

The light separator 323 reflects the light from the observed region 100,which passes through the objective lens 23, to the white light imagingunit 326 and the fluorescent light imaging unit 327. Specifically, thelight separator 323 is configured with a dichroic mirror or a halfmirror. The light separator 323 reflects the light that contains atleast the reflected white light from the observed region 100 and thelight that contains at least the fluorescent light from the observedregion 100, out of the light from the observed region 100 that isconcentrated by the objective lens 23, respectively to the optical pathof the white light imaging unit 326 and to the optical path of thefluorescent light imaging unit 327. For example, if the light separator323 is a dichroic mirror, the light separator 323 reflects the whitelight in the wavelength band less than a predetermined wavelength band(for example, less than 680 nm) out of the light from the observedregion 100 to the optical path of the white light imaging unit 326. Thelight separator 323 reflects the fluorescent light and the excitationlight in the predetermined wavelength band or more (for example, 680 nmor more) to the optical path of the fluorescent light imaging unit 327.The white light of 400 to 680 nm that is reflected from the observedregion 100 can be taken as an example of the white light in thewavelength band less than the predetermined wavelength. For example, theexcitation light of 680 to 740 nm that is reflected from the observedregion 100 and the fluorescent light of 760 to 850 nm that is generatedfrom the observed region 100 can be taken as the excitation light andthe fluorescent light in the predetermined wavelength band or more. Onthe other hand, if the light separator 323 is a half mirror, the lightseparator 323 reflects the light from the observed region 100 to thewhite light imaging unit 326 and the fluorescent light imaging unit 327as in the case of the dichroic mirror.

The barrier filter 324 allows the fluorescent light from the observedregion 100 to pass, and cuts off the light out of the wavelength band ofthe fluorescent light. Specifically, the barrier filter 324 cuts offreflected the light from the observed region 100 (for example, theexcitation light in the wavelength band of 680 to 740 nm), out of thelight from the observed region 100, that is reflected by the lightseparator 323 to the fluorescent light imaging unit 327, and allow thefluorescent light from the observed region 100 (for example, thefluorescent light of 760 to 850 nm) to pass to the fluorescent lightimaging unit 327.

The imaging unit 325 includes the white light imaging unit 326 and thefluorescent light imaging unit 327. The imaging unit 325 has a functionof capturing a white light image of the observed region 100 with thefocus being on the observed region (in the focused state). The imagingunit 325 has a function of capturing the fluorescent light image of theobserved region 100 in the focused state and the adjusted image of theobserved region 100 in the unfocused state at the same timing.

The focus of the white light imaging unit 326 can be controlled by theimage processing device 330 of the controller 339. The white lightimaging unit 326 captures the white light image of the observed region100 with the focus being on the observed region 100, and captures theadjusted image of the observed region 100 after the controller 339controls the focus. Specifically, the white light imaging unit 326includes an imaging device 326 a, such as a CCD or COMS image sensor;and a movable optical system 326 b, such as a lens, for forming anoptical image of the object on the light receiver of the imaging device326 a.

As the imaging device 25 a according to the first embodiment is, theimaging device 326 a is a Bayer color imaging device that includes agroup of color filters with different spectral properties arranged onthe light receiver. The imaging device 326 a receives the reflectedlight from the observed region 100 that is imaged by the movable opticalsystem 326 b via the color filter group, and performs the photoelectricconversion process on the received light with respect to each pixel ofeach color component in order to generate video signals of each colorcomponent of the observed region 100. When the white light from thelight source device 310 is applied to the observed region 100, theimaging device 326 a receives the white light from the observed region100 that is imaged by the movable optical system 326 b via the colorfilter group, thereby capturing the white light image or the adjustedimage of the observed region 100. Each time the imaging device 326 acaptures a white light image or an adjusted image of the observed region100, the imaging device 326 a sequentially transmits video signals ofeach color component constituting the white light image or the adjustedimage of the observed region 100 to the image acquiring unit 31 of theimage processing device 330.

The movable optical system 326 b is configured with an optical system,such as a lens, and a movable lens frame. The movable optical system 326b is driven and controlled by the controller 339 of the image processingdevice 330. Specifically, the movable optical system 326 b is drivenunder the control of the controller 339, so that the relative distancebetween the imaging device 326 a and the lens can be changed. The focusof the movable optical system 326 b is controlled by the controller 339,and thus the movable optical system 326 b can focus on the observedregion 100. With the focus being on the observed region 100, the movableoptical system 326 b images the white light from the observed region100, which is reflected by the light separator 323 to the optical pathof the white light imaging unit 326, on the light receiver of theimaging device 326 a. In this case, the imaging device 326 a capturesthe white light image of the observed region 100 in the focused state.

The movable optical system 326 b can shift the focus from the observedregion 100 at a desired degree under the control of the controller 339.With the focus being shifted from the observed region 100 under thecontrol of the controller 339, the movable optical system 326 b imagesthe white light from the observed region 100, which is reflected by thelight separator 323 to the optical path of the white light imaging unit326, on the light receiver of the imaging device 326 a. In this case,the imaging device 326 a captures the adjusted image that is thefocus-controlled white light image of the observed region 100 after thefocus control. Thereafter, the movable optical system 326 b returns tothe state where it focuses on the observed region 100 under the controlof the controller 39. As in the case of the first embodiment, thegradation level of the adjusted image of the observed region 100 variesdepending on the result of adjusting the focus of the movable opticalsystem 326 b under the control of the controller 339.

The fluorescent light imaging unit 327 is an imaging unit of which focusis fixed. The fluorescent light imaging unit 327 captures thefluorescent light image of the observed region 100 with the focus beingon the observed region 100. Specifically, the fluorescent light imagingunit 327 includes an imaging device 327 a, such as a CCD or a CMOS imagesensor; and an optical system 327 b, such as a lens, for forming afluorescent light image of the object on the light receiver of theimaging device 327 a.

The imaging device 327 a is configured, for example, with a monochromeimaging device with higher sensitivity compared to the imaging device326 a of the white light imaging unit 326, and captures the fluorescentlight image of the observed region 100. The imaging device 327 areceives the fluorescent light from the observed region 100 that isimaged by the optical system 327 b. With the focus being on the observedregion 100, the optical system 327 b images the fluorescent light fromthe observed region 100, which is reflected by the light separator 323to the optical path of the fluorescent light imaging unit 327, and whichpasses through the barrier filter 324, on the light receiver of theimaging device 327 a. The imaging device 327 a performs thephotoelectric conversion process on the fluorescent light with respectto each pixel in order to generate video signals of the observed region100. When the white light from the light source device 310 is applied tothe observed region 100, the imaging device 327 a receives thefluorescent light from the observed region 100 that is imaged by theoptical system 327 b, thereby capturing the fluorescent light image.Each time the imaging device 327 a captures a fluorescent light image ofthe observed region 100, the imaging device 327 a sequentially transmitseach video signal constituting the fluorescent light image of theobserved region 100 to the image acquiring unit 31 of the imageprocessing device 330.

In the third embodiment, the white light imaging unit 326 and thefluorescent light imaging unit 327 capture the images of the observedregion 100 at the same timing under the control of the controller 339.Specifically, the fluorescent light imaging unit 327 captures thefluorescent light image of the observed region 100 at the timing same asthat at which the white light imaging unit 326 captures the white lightimage or the adjusted image of the observed region 100. In other words,the positions of the subject coincide between the adjusted image of theobserved region 100, which is captured by the white light imaging unit326, and the fluorescent light image of the observed region 100, whichis captured by the fluorescent light imaging unit 327.

The image processing device 330 includes the controller 339 as describedabove, and has a control function of causing the white light imagingunit 326 and the fluorescent light imaging unit 327 to perform the imagecapturing operations at the same timing. The image processing device 330has functions similar to those of the image processing device 30according to the first embodiment in addition to the function of thecontroller 339.

The controller 339 is configured with a storage unit 339 a that storespredetermined process programs including an image processing program;and a computer that executes the process programs in the storage unit339 a. The storage unit 339 a is a computer-readable storage mediumaccording to the third embodiment. The controller 339 drives andcontrols the movable optical system 326 b of the white light imagingunit 326 such that it focuses on the observed region 100 based oninstruction information that is input by the input unit 38, and controlsthe imaging device 326 a of the white light imaging unit 326 such thatit captures the white light image of the observed region 100 in thefocused state. The controller 339 also controls the focus of the whitelight imaging unit 326 by driving and controlling the movable opticalsystem 326 b such that it shifts the focus from the observed region 100by the amount of a shift in accordance with the gradation level that isset by the gradation level setting unit 34. The controller 339 controlsthe imaging device 326 a of the white light imaging unit 326 such thatit captures the adjusted image of the observed region 100 in theunfocused state, and controls the imaging device 327 a of thefluorescent light imaging unit 327 such that it captures the fluorescentlight image of the observed region 100 in the focused state at the sametiming at which the adjusted image is captured. Thereafter, thecontroller 339 drives and controls the movable optical system 326 b suchthat it returns to the state where it focuses on the observed region100. The controller 339 has functions similar to those of the controller39 of the image processing device 30 according to the first embodimentin addition to the functions of controlling the white light imaging unit326 and the fluorescent light imaging unit 327.

The controller 339 may control the imaging device 327 a of thefluorescent light imaging unit 327 such that it does not captures thefluorescent light image of the observed region 100 at the timing atwhich the white light imaging unit 326 captures the white light image ofthe observed region 100 in the focused state, or may control the imagingdevice 327 a of the fluorescent light imaging unit 327 such that itcaptures the fluorescent light image of the observed region 100 at thethis timing. When the controller 339 causes the fluorescent lightimaging unit 327 to capture a fluorescent light image at the timing sameas that at which the white light image of the observed region 100 in thefocused state is captured, the controller 339 may control the imageacquiring unit 31 such that it deletes each video signal of thefluorescent light image at this timing.

The operations of the image processing device 330 according to the thirdembodiment of the present invention are explained below. FIG. 10 is aflowchart illustrating the process procedure of the image processingdevice according to the third embodiment of the present invention. Theimage processing device 330 according to the third embodiment performsthe process procedure illustrated in FIG. 10 and displays and outputsthe white light image and the fluorescent light image of the observedregion 100.

In other words, as illustrated in FIG. 10, the image processing device330 first acquires the white light image of the observed region 100(step S201). At step S201, the controller 339 controls the light sourcedevice 310, the imaging system 328, and the image acquiring unit 31 suchthat the white light image of the observed region 100 that is beingfocused is acquired.

Under the control of the controller 339, the light source device 310applies the white light in the wavelength band (for example, 440 to 740nm) covering that of the excitation light to the observed region 100because of the functions of the white light source 311 and the filter314. The white light imaging unit 326 of the imaging system 328 focuseson the observed region 100 because the controller 339 drives andcontrols the movable optical system 326 b. With the focus being on theobserved region 100, the white light imaging unit 326 receives the whitelight that is reflected from the observed region 100, thereby capturingthe white light image of the observed region 100. Under the control ofthe controller 339, the image acquiring unit 31 acquires each videosignal of the white light image of the observed region 100 from thewhite light imaging unit 326, and transmits each acquired video signalto the storage unit 32 and the feature value calculator 33 afterperforming the above-described signal processes. The storage unit 32stores each video signal of the white light image of the observed region100 that is acquired from the image acquiring unit 31.

At step 201, the controller 339 may control the fluorescent lightimaging unit 327 such that it does not capture the fluorescent lightimage of the observed region 100, or control the fluorescent lightimaging unit 327 such that it captures the fluorescent light image ofthe observed region 100. When the controller 339 causes the fluorescentlight imaging unit 327 to capture the fluorescent light image of theobserved region 100 at step S201, the controller 339 may control theimage acquiring unit 31 such that it deletes each video signal of thefluorescent light image or may cause the image output unit 37 such thatit displays and outputs the fluorescent light image.

The image processing device 330 then performs the process forcalculating the feature value of the white light image of the observedregion 100 that is acquired at step S201 (step S202) as at step S103according to the first embodiment illustrated in FIG. 4. Subsequently,the image processing device 330 performs the process for setting thegradation level of the white light image of the observed region 100 onthe basis of the feature value calculated at step S202 (step S203) as atstep S104 of the first embodiment illustrated in FIG. 4.

At step S202 and step S203, as in the case of the first embodiment, thecontroller 339 controls the feature value calculator 33 such that itcalculates the feature value of the white light image of the observedregion 100 that is acquired at step S201 and then controls the gradationlevel setting unit 34 such that it sets the gradation level of the whitelight image of the observed region 100 on the basis of the feature valuecalculated at step S202.

Subsequently, the image processing device 330 performs the process foradjusting the focus on the white light image of the observed region 100on the basis of the gradation level set at step S203 (step S204). Atstep S204, the controller 339 calculates the amount of shift of thefocus from the observed region 100 on the basis of the gradation levelof the white light image of the observed region 100 that is set by thegradation level setting unit 34. The controller 339 controls the focusof the imaging system 328 such that the focus is shifted from theobserved region 100 by the calculated shift amount.

Under the control of the controller 339, the white light imaging unit326 of the imaging system 328 changes the relative distance between theimaging device 326 a and the lens of the movable optical system 326 band shifts the focus from the observed region 100 by the shift amount inaccordance with the gradation level that is set by the gradation levelsetting unit 34. At step S204, the fluorescent light imaging unit 327keeps focusing on the observed region 100.

The image processing device 330 acquires the fluorescent light image ofthe observed region 100 and the adjusted image that is the white lightimage of the observed region 100 after the control of the focus on theobserved region 100 in accordance with the set gradation level (stepS205). At step S205, the controller 339 controls the light source device310, the imaging system 328, and the image acquiring unit 31 such thatthe fluorescent light image of the observed region 100 being focused isacquired and the adjusted image of the observed region 10 not beingfocused in accordance with the gradation level set at step S203 isacquired.

Under the control of the controller 339, the light source device 310applies again the white light in the wavelength band (for example, 400to 740 nm) covering that of the excitation light to the observed region100 because of the functions of the white light source 311 and thefilter 314. With the focus being on the observed region 100, thefluorescent light imaging unit 327 of the imaging system 328 receivesthe fluorescent light, which is generated from the observed region 100due to the excitation light in the white light, thereby capturing thefluorescent light image of the observed region 100. Simultaneously, withthe focus that is adjusted at step S204, the white light imaging unit326 of the imaging system 328 receives the white light that is reflectedfrom the observed region 100, thereby capturing the adjusted image ofthe observed region 100. Under the control of the controller 339, theimage acquiring unit 31 acquires each video signal of the fluorescentlight image of the observed region 100, which is capture by thefluorescent light imaging unit 327, and each video signal of theadjusted image of the observed region 100, which is captured by thewhite light imaging unit 326 at the same timing as that of thefluorescent light image. The image acquiring unit 31 transmits eachvideo signal, which is acquired from the fluorescent light imaging unit327, to the storage unit 32 after performing the above-described signalprocesses. The storage unit 32 stores each video signal of thefluorescent light image, which is acquired from the image acquiring unit31. The image acquiring unit 31 also transmits each video signal of theadjusted image of the observed region 100, which is acquired from thewhite light imaging unit 326, to the corrected fluorescent light imagegenerator 35 after performing the above-described processes.

Subsequently, the image processing device 330 performs the process forgenerating the fluorescent light image of the observed region 100 (stepS206) as at step S107 according to the first embodiment illustrated inFIG. 4, and then performs the process for outputting the imageinformation of the observed region 100 (step S207) as at step S108according to the first embodiment illustrated in FIG. 4.

At step S206, as in the case of the first embodiment, the controller 339controls the corrected fluorescent light image generator 35 such that itgenerates the corrected fluorescent light image that is obtained bycorrecting the luminance value of the fluorescent light image of theobserved region 100. At step S207, as in the case of the firstembodiment, the controller 339 then controls the white light imagegenerator 36 such that it generates the white light image of theobserved region 100, and controls the image output unit 37 such that itdisplays the white light image and the corrected fluorescent light imageof the observed region 100 to be output.

Thereafter, when a process completion operation, such as a predeterminedoff operation, is performed (YES at step S208) as at step S109 accordingto the first embodiment illustrated in FIG. 4, the image processingdevice 330 completes the process. In contrast, when the processcompletion operation is not performed (NO at step S209), the imageprocessing device 330 goes back to step S201 and repeats the processprocedure from step S201 and the following steps. In this case, thecontroller 339 performs the process procedure from step S201 to stepS208 in order to appropriately control the light source device 310, theimaging system 328, and each constituent of the image processing device330.

The adjusted image and the fluorescent light image of the observedregion 100 that are acquired at step S205 are the information of theimages of the observed region 100 that are captured at the same timingby the white light imaging unit 326 and the fluorescent light imagingunit 327. Thus, the positions of the object, such as the lesion 101,coincide between the adjusted image and the fluorescent light image. Thecorrected fluorescent light image generator 35 divides the luminancevalue of the fluorescent light image of the observed region 100 by theluminance value of the adjusted image, containing the object of whichposition coincides with that in the fluorescent light image, withrespect to each corresponding pixel. Accordingly, the luminance value ofthe fluorescent light image of the observed region 100 can be correctedwith high accuracy without influence of the shift of the position of theobject between the images. As a result, the corrected fluorescent lightimage generator 35 can generate the corrected fluorescent light image ofthe observed region 100 that is obtained by correcting the luminancevalue with higher accuracy compared with the first embodiment.

As explained above, in the third embodiment, the fluorescent light imageof the observed region to be observed and the adjusted image, which isthe white light image of the observed region 100 captured after thefocus control at the same timing at which the fluorescent light image iscaptured, are acquired first. By performing division regarding thefluorescent light image of the observed region using the acquiredadjusted image, the corrected fluorescent light image, which is obtainedby correcting the luminance value of the fluorescent light image, isgenerated. Other aspects of the configuration are similar to those ofthe first embodiment. Thus, functions and effects similar to those ofthe first embodiment can be achieved, and the positions of the objectcan coincide between the fluorescent light image and the adjusted imagethat are used for generating the corrected fluorescent light image. Byuse of the adjusted image containing the object of which positioncoincides with that in the fluorescent light image to be corrected, theluminance value of the fluorescent light image of the observed regioncan be corrected with higher accuracy without influence of the shift ofthe position of the object between the images.

By use of the image processing device, the imaging device, thecomputer-readable storage medium, and the image processing methodaccording to the third embodiment, abnormal body tissue, such as alesion, in the observed region can be drawn clearly with high accuracyin the fluorescent light image. This further increase the capability ofdetecting abnormal body tissue in the subject, using the fluorescentlight image to be observed, and increases the diagnostic capability.

In the first embodiment, the feature value calculator 33 calculates thetotal luminance value of the entire edge, which is contained in thewhite light image of the observed region 100, as the edge informationthat is an example of the feature value of the white light image.Alternatively, the feature value calculator 33 may calculate the numberof pixels of the entire edge, which is contained in the white lightimage of the observed region 100, as the edge information of the whitelight image. Alternatively, the feature value calculator 33 maypreviously stores a threshold relating to the pixel luminance value,compare each luminance value of the entire edge contained in the whitelight image of the observed region 100 with the threshold, and calculatethe number of pixels of the edge with the luminance value of thethreshold or higher as the edge information of the white light image. Inthis case, the data table 34 a contains each range of the number ofpixels serving as the edge information and gradation level that differsin each range of the number of pixels. With reference to the data table34 a, the gradation level setting unit 34 increases the gradation levelof the white light image with an increase of the number of pixelsserving as the edge information, and decreases the gradation level ofthe white light image with a decrease in the number of pixels serving asthe edge information.

In the second embodiment, the feature value calculator 233 calculatesthe dispersion, which represents the degree of dispersion of thedetected color component (for example, the red color component) in thewhite light image of the observed region 100, as the color distributioninformation that is an example of the feature value of the white lightimage. Alternatively, the feature value calculator 233 may calculate thepeak-to-peak difference of the color distribution in the white lightimage as the color distribution information of the white light image ofthe observed region 100. FIG. 11 is a schematic diagram representing anexample of the peak-to-peak difference of the color distribution in thewhite light image of the observed region. Specifically, the featurevalue calculator 233 detects the color distribution of the predeterminedcolor component (for example, the red color component) in the whitelight image of the observed region 100 and other color components (forexample, the blue color component and the green color component) in thewhite light image on the basis of each video signal of the white lightimage. The feature value calculator 233 calculates a peak-to-peakdifference ΔP (see FIG. 11) of the detected color distribution as thecolor distribution information of the white light image. In this case,the data table 234 a contains each range of the peak-to-peak differenceΔP serving as the color distribution information and the gradation levelthat differs in each range of the peak-to-peak difference ΔP. Withreference to the data table 234 a, the gradation level setting unit 234reduces the gradation level of the white light image with an increase ofthe peak-to-peak difference ΔP serving as the color distributioninformation, and increases the gradation level of the white light imagewith a decrease of the peak-to-peak difference ΔP serving as the colordistribution information.

The peak-to-peak difference ΔP of the detected color distributionrepresents the degree of concentration of the predetermined color in thewhite light image of the observed region 100. The degree of dispersion(dispersion) of the predetermined color in the white light imageincrease with an increase of the peak-to-peak difference ΔP, and thedegree of concentration of the predetermined color in the white lightimage increases with a decrease of the peak-to-peak difference ΔP.

In the second embodiment, the case where the red color component isdetected as the color distribution information of the white light imageof the observed region 100. Alternatively, the color component that isdetected as the color distribution information of the white light imageof the observed region 100 may be the blue color component other thanthe red color component, for example, the greed color component, orother color components such as yellow, cyan, and magenta.

In the first and second embodiment, the fluorescent light image of theobserved region 100 is acquired at step S102 illustrated in FIG. 4.Alternatively, the fluorescent light image of the observed region 100 tobe corrected may be acquired at desired timing as long as the timing isbefore the process procedure of step S105, i.e., before the focus of theimaging unit 25 is shifted from the observed region 100.

Furthermore, in the first to third embodiments, the gradation levelsetting unit includes the data table that represents the relationbetween the feature value and the gradation level of the white lightimage, and sets the gradation level of the white light image withreference to the data table. Alternatively, the gradation level settingunit may previously store an equation of the gradation level thatrepresents the relation between the feature value (for example, the edgeinformation or the color distribution information of the white lightimage), which is calculated by the feature value calculator, and thegradation level of the white light image and may calculate the gradationlevel of the white light image on the basis of the equation of thegradation level and the feature value of the white light image that isacquired from the feature value calculator.

In the first to third embodiments, the controller calculates the amountof shift of the focus from the observed region on the basis of thegradation level of the white light image that is set by the gradationlevel setting unit. Alternatively, the process for calculating the shiftamount of the focus, which is performed by the controller, may be basedon an equation that is previously set in the controller, or may be basedon a lookup table that is previously set in the controller.

In the first to third embodiments, the white light image of the observedregion is generated in the process procedure at step S108 illustrated inFIG. 4 or step S207 illustrated in FIG. 10, i.e., in the process foroutputting the image information of the observed region. Alternatively,the white light image of the observed region may be generated in adesired process procedure from capturing of the white light image untiloutputting of the white light image.

In the first to third embodiments, the focus on the observed region iscontrolled by driving and controlling the movable optical system.Alternatively, the focus on the observed region may be controlled bycontrolling the relative distance between the imaging device and thelens. For example, the focus on the observed region 100 may becontrolled by fixing the optical system, such as a lens, and moving theimaging device in parallel, or may be controlled by moving both of themovable optical system and the imaging device.

In the first and second embodiments, the white light image and thefluorescent light image of the observed region 100 are capturedalternately by the single imaging unit 25. Alternatively, a white lightimaging unit, a fluorescent light imaging unit, and a light separator asthose illustrated in the third embodiment may be arranged in theinsertion unit 20, and a white light image and a fluorescent light imageof the observed region 100 may be captured alternately by the whitelight imaging unit and the fluorescent light imaging unit. In this case,the wavelength band of the excitation light that is applied to theobserved region 100 is not limited to that of visible light or less.Alternatively, the wavelength may be within the wavelength band ofvisible light or may be the wavelength band of visible light or more.

In the third embodiment, the edge information is calculated as thefeature value of the white light image as in the case of the firstembodiment. Alternatively, the color distribution information may becalculated as the feature value of the white light image as in the caseof the second embodiment. In this case, it suffices that the imageprocessing device 330 according to the third embodiment includes thefeature value calculator 233 according to the second embodiment insteadof the feature value calculator 33 and includes the gradation levelsetting unit 234 according to the second embodiment instead of thegradation level setting unit 34.

In the first to third embodiments, the white light is applied as anexample of illuminating light to the observed region. Alternatively, theilluminating light that is applied to the observed region may be lightof a desired color component, for example, red or green.

In the first to third embodiments, the white light image and thecorrected fluorescent light image of the observed region are displayedon the image output unit 37. Alternatively, the image output unit 37 maybe a printer that prints the white light image and the fluorescent lightimage of the observed region on a printing medium, such as paper.Alternatively, the image output unit 37 may be a storage device thatincludes a built-in storage medium, such as a hard disk, or a portablestorage medium, such as a memory card, and that stores the white lightimage and the fluorescent light image of the observed region in thestorage medium.

In the first to third embodiments, the endoscope for observing asubject, such as body tissue, is explained as the imaging deviceaccording to the present invention. Alternatively, the imaging deviceaccording to the present invention may be an endoscope or a microscopethat is used in fields other then the medical field. Alternatively, theimaging device may be an imaging device other than endoscopes andmicroscopes, such as a digital camera or a digital video camera, or aportable information terminal device, such as a mobile phone with animaging function. The image processing device according to the presentinvention is not limited to those incorporated in medical endoscopes.The image processing device may be incorporated in an endoscope or amicroscope used in fields other than the medical field. Alternatively,the image processing device may be incorporated in imaging devices, suchas a digital camera or a digital video camera, other than endoscopes ormicroscopes used in the medical field, or may be incorporated in aportable information terminal device, such as a mobile phone with animaging function.

In the first to third embodiments, the process procedure of the imageprocessing device by software based on operations of the controller thatexecutes the process programs is explained. Alternatively, the imageprocessing device according to the present invention may perform theprocess procedure by hardware. The computer readable storage medium ofeach embodiment stores the image processing program for performing theimage processing according to the image processing method of theembodiment. The storage medium may be a built-in storage medium, such asa hard disk, or a portable storage medium, such as a memory card.

The image processing devices, the imaging devices, the computer readablestorage media, and the image processing methods according to theembodiments accurately correct the luminance of the fluorescent lightimage of the observed region without influence of the line object in theobserved region.

Additional advantages and modifications will readily occur to thoseskilled in the art. Therefore, the invention in its broader aspects isnot limited to the specific details and representative embodiments shownand described herein. Accordingly, various modifications may be madewithout departing from the spirit or scope of the general inventiveconcept as defined by the appended claims and their equivalents.

What is claimed is:
 1. An image processing device, comprising: an imageacquiring unit that acquires a reflected light image and a fluorescentlight image of an observed region, the reflected and the fluorescentlight images being captured by an imaging system; a feature valuecalculating unit that calculates a feature value of the reflected lightimage acquired by the image acquiring unit; an amount-of-shift-of-focussetting unit that sets an amount of shift of focus of the reflectedlight image on the basis of the feature value; a control unit thatcontrols a focus of the imaging system in accordance with the amount ofshift of focus; and a corrected fluorescent light image generating unitthat generates a corrected fluorescent light image that is obtained bycorrecting the fluorescent light image using a focus-controlledreflected light image of the observed region that is acquired by theimage acquiring unit when the focus is controlled to be in an unfocusedstate; wherein the control unit controls the focus so that thefocus-controlled reflected light image arrives at the unfocused state inthe observed region in accordance with the amount of shift of focus;wherein the imaging system includes: a reflected light imaging unit thatcaptures the reflected light image with the focus being on the observedregion; and a fluorescent light imaging unit that captures thefluorescent light image with the focus being on the observed region;wherein the reflected light imaging unit captures the focus-controlledreflected light image after the focus is controlled in accordance withthe amount of shift of focus.
 2. The image processing device accordingto claim 1, wherein the corrected fluorescent light image generatingunit performs division regarding the fluorescent light image, using thefocus-controlled reflected light image of the observed region, in orderto generate the corrected fluorescent light image that is obtained bycorrecting a luminance value of the fluorescent light image.
 3. Theimage processing device according to claim 1, wherein the feature valueis edge information of the reflected light image.
 4. The imageprocessing device according to claim 3, wherein theamount-of-shift-of-focus setting unit increases the amount of shift offocus of the reflected light image with an increase of the edgeinformation, and reduces the amount of shift of focus of the reflectedlight image with a decrease of the edge information.
 5. The imageprocessing device according to claim 1, wherein the feature value is adispersion of a predetermined color component that is contained in thereflected light image.
 6. The image processing device according to claim5, wherein the amount-of-shift-of-focus setting unit increases theamount of shift of focus of the reflected light image with an increaseof the dispersion of the predetermined color component, and reduces theamount of shift of focus of the reflected light image with a decrease ofthe dispersion of the predetermined color component.
 7. The imageprocessing device according to claim 1, wherein the feature value is apeak-to-peak difference of a color distribution of the reflected lightimage.
 8. The image processing device according to claim 7, wherein theamount-of-shift-of-focus setting unit increases the amount of shift offocus of the reflected light image with an increase of the peak-to-peakdifference of the color distribution, and reduces the amount of shift offocus of the reflected light image with a decrease of the peak-to-peakdifference of the color distribution.
 9. The image processing deviceaccording to claim 1, wherein the imaging system sequentially capturesthe reflected light image and the fluorescent light image with the focusbeing on the observed region, and captures the focus-controlledreflected light image after the focus is controlled in accordance withthe amount of shift of focus.
 10. The image processing device accordingto claim 1, wherein the fluorescent light imaging unit captures thefluorescent light image at the same timing as that at which thefocus-controlled reflected light image is captured.
 11. An imagingdevice, comprising: a light source that applies illuminating light andexcitation light to an observed region; an imaging system that capturesa reflected light image of the observed region based on the illuminatinglight that is reflected from the observed region, and captures afluorescent light image of the observed region based on fluorescentlight that is generated from the observed region due to application ofthe excitation light; a feature value calculating unit that calculates afeature value of the reflected light image that is captured by theimaging system; an amount-of-shift-of-focus setting unit that sets anamount of shift of focus of the reflected light image on the basis ofthe feature value; a control unit that controls the focus of the imagingsystem in accordance with the amount of shift of focus; and a correctedfluorescent light image generating unit that generates a correctedfluorescent light image that is obtained by correcting the fluorescentlight image using a focus-controlled reflected light image of theobserved region that is acquired by the image acquiring unit when thefocus is controlled to be in an unfocused state; wherein the controlunit controls the focus so that the focus-controlled reflected lightimage arrives at the unfocused state in the observed region inaccordance with the amount of shift of focus; wherein the imaging systemincludes: a reflected light imaging unit that captures the reflectedlight image with the focus being on the observed region; and afluorescent light imaging unit that captures the fluorescent light imagewith the focus being on the observed region; wherein the reflected lightimaging unit captures the focus-controlled reflected light image afterthe focus is controlled in accordance with the amount of shift of focus.12. A non-transitory computer-readable storage device that storestherein an image processing program that contains instructions forcausing a computer to perform: acquiring a reflected light image and afluorescent light image of an observed region; calculating a featurevalue of the reflected light image; setting an amount of shift of focusof the reflected light image on the basis of the feature value;controlling a focus of an imaging system for the reflected light imagein accordance with the amount of shift of focus; acquiring afocus-controlled reflected light image of the observed region when thefocus is controlled to be in an unfocused state; and generating acorrected fluorescent light image that is obtained by correcting thefluorescent light image of the observed region, using thefocus-controlled reflected light image that is in the unfocused state;wherein the focus of the imaging system is controlled so that thefocus-controlled reflected light image arrives at the unfocused state inthe observed region in accordance with the amount of shift of focus;wherein the instructions further cause the computer to perform:capturing, with a reflected light imaging unit of the imaging system,the reflected light image with the focus being on the observed region;and capturing, with a fluorescent light imaging unit of the imagingsystem, the fluorescent light image with the focus being on the observedregion; wherein the reflected light imaging unit captures thefocus-controlled reflected light image after the focus is controlled inaccordance with the amount of shift of focus.
 13. An image processingmethod, comprising: acquiring a reflected light image and a fluorescentlight image of an observed region; calculating a feature value of thereflected light image; setting an amount of shift of focus of thereflected light image on the basis of the feature value; controlling afocus of an imaging system for the reflected light image in accordancewith the amount of shift of focus; acquiring a focus-controlledreflected light image of the observed region when the focus iscontrolled to be in an unfocused state; and generating a correctedfluorescent light image that is obtained by correcting the fluorescentlight image of the observed region, using the focus-controlled reflectedlight image that is in the unfocused state; wherein the focus of theimaging system is controlled so that the focus-controlled reflectedlight image arrives at the unfocused state in the observed region inaccordance with the amount of shift of focus; wherein the method furthercomprises: capturing, with a reflected light imaging unit of the imagingsystem, the reflected light image with the focus being on the observedregion; and capturing, with a fluorescent light imaging unit of theimaging system, the fluorescent light image with the focus being on theobserved region; wherein the reflected light imaging unit captures thefocus-controlled reflected light image after the focus is controlled inaccordance with the amount of shift of focus.
 14. An image processingdevice, comprising: a processor; and a memory storing computer readableinstructions that, when executed by the processor, implement: an imageacquiring unit that acquires a reflected light image and a fluorescentlight image of an observed region, the reflected and the fluorescentlight images being captured by an imaging system; a feature valuecalculating unit that calculates a feature value of the reflected lightimage acquired by the image acquiring unit; an amount-of-shift-of-focussetting unit that sets an amount of shift of focus of the reflectedlight image on the basis of the feature value; a control unit thatcontrols a focus of the imaging system in accordance with the amount ofshift of focus; and a corrected fluorescent light image generating unitthat generates a corrected fluorescent light image that is obtained bycorrecting the fluorescent light image using a focus-controlledreflected light image of the observed region that is acquired by theimage acquiring unit when the focus is controlled to be in an unfocusedstate; wherein the control unit controls the focus so that thefocus-controlled reflected light image arrives at the unfocused state inthe observed region in accordance with the amount of shift of focus;wherein the imaging system includes: a reflected light imaging unit thatcaptures the reflected light image with the focus being on the observedregion; and a fluorescent light imaging unit that captures thefluorescent light image with the focus being on the observed region;wherein the reflected light imaging unit captures the focus-controlledreflected light image after the focus is controlled in accordance withthe amount of shift of focus.